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BioMed Research International
Volume 2015, Article ID 876047, 13 pages
http://dx.doi.org/10.1155/2015/876047
Research Article

Putative Epimutagens in Maternal Peripheral and Cord Blood Samples Identified Using Human Induced Pluripotent Stem Cells

1Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, The University of Tokyo, Tokyo 113-8657, Japan
2Laboratory of Genomic Function Engineering, Department of Life Science, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan
3Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan
4Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo 142-8501, Japan
5Division of Food Chemistry, Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan
6Saitama Prefectural Institute of Public Health, Saitama 355-0133, Japan
7Department of Toxicology, Aichi Prefectural Institute of Public Health, Aichi 462-8576, Japan
8Department of Obstetrics and Gynecology, School of Medicine, Tokai University, Kanagawa 259-1193, Japan
9Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan
10Department of Pharmacology, School of Medicine, Aichi Medical University, Aichi 480-1195, Japan
11Faculty of Home Economics, Otsuma Women’s University, Tokyo 102-8357, Japan
12Fuji-Oyama Hospital, Shizuoka 410-1326, Japan

Received 15 November 2014; Revised 26 January 2015; Accepted 9 March 2015

Academic Editor: Francesca Pacchierotti

Copyright © 2015 Yoshikazu Arai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1. Detection of living and dividing cells based on measurement of M phase nuclei. The numbers of M phase nuclei in the images of DAPI staining that were used to detect heterochromatin dot after single or combined exposure to the chemicals in Figs. 2-4. For each sample, 5-10 images from two independent experiments were used to count M phase nuclei in chemical-exposed and in solvent-exposed control cells. Relative value (mean ± SEM) of the number of M phase nuclei in chemical-exposed cells were calculated based on the number of M phase nuclei in control cells. 1×, serum level detected in cord blood samples and/or pregnant mothers’ serum; 10×, ten-fold higher level than that of cord blood samples and/or pregnant mothers’ serum; Epimutagen mixture, combined exposure to all five chemicals (DEP, Hg, cotinine, Se, and S-421) at the serum level.

  1. Supplementary Figures