BioMed Research International

Research Article

Fuzheng Huayu Recipe Ameliorates Liver Fibrosis by Restoring Balance between Epithelial-to-Mesenchymal Transition and Mesenchymal-to-Epithelial Transition in Hepatic Stellate Cells

Table 1

Primers for real-time PCR.
GenePrimer sequence (5′-3′)Annealing temperature (°C)Product length (bp)
ALBForward primer:
GACTGCCCTGTGTGGAAGAC
Reverse primer:
CGAAGTCACCCATCACCGTC		56321
-SMAForward primer:
GCGGGGATCCATGAGACCACCTAC
Reverse primer:
GAGCCGCCGATCCACACTGA		60218
CK19Forward primer:
CACTACGCAGATCCAGATAAACA
Reverse primer:
GAAGTCGCACTGGTAGCAAG		56388
CD31Forward primer:
CTTCACCATCCAGAAGGAAGAGAC
Reverse primer:
CACTGGTATTCCATGTCTCTGGTG		56360
CD68Forward primer:
TCATGGGAATGCCACAGTTTC
Reverse primer:
GAGGGCCAACAGTGGAGAA		5662
DesminForward primer:
GGCCGACGCAGTGAACCAG
Reverse primer:
CGCCGCAGCTCTCGCATCT		60213
E-cadherinForward primer:
TGAAGCCCCCATTTTTGTGC
Reverse primer:
GCTCCGAATCTTCTCTGTCCATCT		58 221
TGF-β1Forward primer:
GCGCCTGCAGAGATTCAAGTCA
Reverse primer:
AAAGCCCTGTATTCCGTCTCCT		56 187
GAPDHForward primer:
CACGGCAAGTTCAACGGCACAGT
Reverse primer:
AGCGGAAGGGGCGGAGATGAT		60 222