Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 948297, 6 pages
http://dx.doi.org/10.1155/2015/948297
Research Article

A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences-Inspection and Testing Center for Environmental Risk Assessment of Genetically Modified Plant-Related Microorganism (Beijing), Ministry of Agriculture, No. 12 Zhongguancun South Street, Haidian District, Beijing 100081, China

Received 10 June 2015; Revised 30 July 2015; Accepted 23 August 2015

Academic Editor: Aspasia Nisiotou

Copyright © 2015 Liang Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rice is one of the most important food crops in the world. Genetically modified (GM) technology has been used in rice to confer herbicide tolerance and pathogen or insect resistance. China invests heavily in research on GM rice. By the end of 2014, at least 250 transgenic rice lines had been developed in China. To monitor the presence of GM rice in food and feed, we collected information on foreign elements from 250 transgenic rice lines and found 5 elements, including the Agrobacterium tumefaciens nopaline synthase terminator (T-NOS), the cauliflower mosaic virus 35S promoter (CaMV35S), the ubiquitin gene (Ubi), the bar gene, and the hygromycin phosphotransferase gene (Hpt), that are commonly present in GM rice. Therefore, we constructed a novel plasmid (pBJGMM001) that contains fragments of these elements and two endogenous reference genes (the sucrose phosphate synthase gene, SPS, and the phosphoenolpyruvate carboxylase gene, PEPC). pBJGMM001 can serve as a standard for detecting 96% of GM rice lines in China. The primers, amplicons, reaction mixture, and PCR program were developed based on Chinese National Standards. The protocol was validated and determined to be suitable for practical use in monitoring and identifying GM rice.