Transcriptional Changes Associated with Long-Term Left Ventricle Volume Overload in Rats: Impact on Enzymes Related to Myocardial Energy Metabolism

Figure 2

(a) Evolution of LV remodeling in experimental volume overload from severe aortic valve regurgitation in Wistar rats after 2 (AR2) and 14 days (AR14). Indexed heart weights were corrected for tibial length whereas relative wall thickness (RWT) was evaluated by echocardiography before sacrifice. Results are reported as the mean ± SEM (–8 per group). between sham and AR groups. (b) LV myocardial activity levels of enzymes implicated in fatty acid β-oxidation, glucose metabolism, and mitochondrial energy production in 9-month AR rats relative to controls. HADH (hydroxyacyl-Coenzyme A dehydrogenase), HK (hexokinase), and citrate synthase (CS) enzymatic activities were measured in LV homogenates from at least 6 animals in each group as described in Section 2. Results are reported in μmoles/min/mg of tissue or as the ratio of HADH/HK activities arbitrarily fixed at 1 for sham group. , , and between groups. (c) LV expression of the 16 genes studied in Figure 1(b) in AR rats at 2 and 14 days. Levels in sham animals were fixed to 1 (Line). versus sham animals and between 2-day AR rats and 14-day animals. (d) PPARα, PGC1α, and RXRs LV mRNA levels in 2- and 14-day AR rats. and compared to sham controls (Line at 1).

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