Molecular Characterization of LRB7 Gene and a Water Channel Protein TIP2 in Chorispora bungeana

Table 1

DNA/RNA primer sequences used in this study.

Primers	From 5′ to 3′	Denote
P1	TTGCTGGDGTTGGGTCHGC	For the first fragment of LRB7 cDNA
P2	TCCGCGGCYGTKGCRTAAACI	For the first fragment of LRB7 cDNA
P3	CGCTTTGGTCTACACCGTCTA	For the second fragment of LRB7 cDNA
P4	TCGGAAGAACMCAYGAASACAT	For the second fragment of LRB7 cDNA
P5	GCGGAATTCATGGCAGGAGTAGCCTTCGGT	For the genomic region of LRB7
P6	GCGAAGCTTGAAATCAGAAGAA GCAAGAGG	For the genomic region of LRB7
P7	GGAGCTGAGAGATTCCGTTGC	For the actins gene
P8	GAAG CATTTCCTGTGGACAATCGA	For the actins gene
P9	ATCTTCTTCATTAAACAAAAAC	Semiquantitative RT-PCR analysis of LRB7
P10	GTAAACGGTGTAGACCAAA	Semiquantitative RT-PCR analysis of LRB7
5′ GSP1	GAGATGTTGGCTCCGATTGCGAC	For the 5′ RACE of LRB7
5′ GSP2	CTAGTCCGGGCGTGTCAAGAGCA	For the 5′ RACE of LRB7
3′ GSP3	TCACTGGGTCTACTGGGTGGGTC	For the 3′ RACE of LRB7
3′ GSP4	TGCCGGAGCTGTTTATGGCAATG	For the 3′ RACE of LRB7