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BioMed Research International
Volume 2016, Article ID 3938523, 8 pages
Research Article

Comparison of Neuroprotective Effect of Bevacizumab and Sildenafil following Induction of Stroke in a Mouse Model

1Department of Neurosurgery, Rabin Medical Center-Beilinson Hospital, 49100 Petach Tikva, Israel
2Krieger Eye Research Laboratory, Felsenstein Medical Research Center, 49202 Petach Tikva, Israel
3Sackler Faculty of Medicine, Tel Aviv University, 6997801 Tel Aviv, Israel
4Pediatric Ophthalmology Unit, Schneider Children’s Medical Center of Israel, 49100 Petach Tikva, Israel

Received 9 January 2016; Revised 11 April 2016; Accepted 21 April 2016

Academic Editor: Monica Fedele

Copyright © 2016 Ivan Novitzky et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To evaluate the effect of bevacizumab and sildenafil on stroke parameters in a mouse model, middle cerebral artery occlusion was induced in male C57Bl/6 mice using an intra-arterial filament method. The filament was removed after 60 minutes, and the mice were immediately given a single intraperitoneal injection of saline, bevacizumab, or sildenafil. An additional group of mice () received bevacizumab 6 h after MCAO induction. The mice were euthanized 24 hours later and evaluated for infarct area and brain edema using triphenyltetrazolium chloride staining and ImageJ. In the saline-treated mice (), total stroke volume was  mm3, mean penumbra area was  mm3, and hemispheric asymmetry was 106.5%. Corresponding values in the bevacizumab group () were  mm3,  mm3, and 108.6%; in the delayed (6 h) bevacizumab injected mice () they were  mm3,  mm3, and 98.2%; and in the sildenafil group () they were  mm3,  mm3, and 109.9%. The bevacizumab group had a significantly larger mean penumbra area when given immediately and smaller total stroke area in both groups than the saline- () and sildenafil-treated () groups. Only delayed bevacizumab group had reduced edema. Bevacizumab, injected immediately or delayed after injury, exerts a neuroprotective/salvage effect, whereas immediate treatment with sildenafil does not. Inflammation may play a role in the neuroprotective effect.