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BioMed Research International
Volume 2016, Article ID 4651265, 11 pages
http://dx.doi.org/10.1155/2016/4651265
Research Article

Proliferation-Related Activity in Endothelial Cells Is Enhanced by Micropower Plasma

1Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-Aoba, Aoba, Sendai 980-8579, Japan
2Institute of Fluid Science, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan

Received 16 September 2016; Accepted 20 November 2016

Academic Editor: Hideaki Fujita

Copyright © 2016 Kotaro Suzuki and Daisuke Yoshino. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Nonthermal plasma has received a lot of attention as a medical treatment technique in recent years. It can easily create various reactive chemical species (ROS) and is harmless to living body. Although plasma at gas-liquid interface has a potential for a biomedical application, the interactions between the gas-liquid plasma and living cells remain unclear. Here, we show characteristics of a micropower plasma with 0.018 W of the power input, generated at gas-liquid interface. We also provide the evidence of plasma-induced enhancement in proliferation activity of endothelial cells. The plasma produced H2O2, HNO2, and HNO3 in phosphate buffered saline containing Mg++ and Ca++ (PBS(+)), and their concentration increased linearly during 600-second discharge. The value of pH in PBS(+) against the plasma discharge time was stable at about 7.0. Temperature in PBS(+) rose monotonically, and its rise was up to 0.8°C at the bottom of a cell-cultured dish by the plasma discharge for 600 s. Short-time treatment of the plasma enhanced proliferation activity of endothelial cells. In contrast, the treatment of H2O2 does not enhance the cell proliferation. Thus, the ROS production and the nuclear factor-kappa B (NF-κB) activation due to the plasma treatment might be related to enhancement of the cell proliferation. Our results may potentially provide the basis for developing the biomedical applications using the gas-liquid plasma.