Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico

Table 3

Primer details for host mammals specific rabies virus variants detection in Mexico.

	Primer name	Sequence (3′ to 5′)	Sense	Rabies variant detection	Fragment size (pb)	Tm (°C)	Length (pb)
E	VAMPIROF	TTCAAGGTCAATAATCAGGTGGTCTCTC	F	Vampire bat	836	59	22–49
	VAMPIRORC	AGACTGCTGTTCCTCATTCCTATTT	R			53.8	833–857
I	FBVFQ	ATTGGGCTCTAACAGGGGGCAT	F		177	59.6	356–377
	FBVRCQ	ATAGAGCAGATTTTCGAGACAGCCCCCT	R			62.5	575–602
E	PERROF	TTCAAAGTCAATAATCAGGTGGTC	F	Dog	1288	51.9	22–45
	PERRORC	AATCATCAAGCCCGTCCAAACT	R			56.4	1288–1309
I	FBPFQ	CAAGAATATGAGGCGGCTGAACT	F		212	55	1099–1120
	PERRORC	AATCATCAAGCCCGTCCAAACT	R			56.4	1288–1309
E	MURCIELAGOF	GACCCTGATGATGTATGCTCTTAT	F	Bat	668	51	196–219
	MURCIELAGORC	GTTCCTCACTCYTATTTCATCCA	R			50.6	742–764
I	FBMFQ	GCTTGACCCTGATGATGTATGCTCTTAT	F		184	59	192–219
	FBMRCQ	TGGGCTCTAACAGGGGGTATGG	R			58.7	358–379
E	ZORRILLOF	ATAGAACAGATTTTTGAGACGGC	F	Skunk	794	51.4	505–527
	ZORRILLORC	TGTCTCAGTTAGTTCCAATCATCAAGC	R			56.9	1272–1298
I	ZORRILLOF	ATAGAACAGATTTTTGAGACGGC	F		359	51.4	506–527
	FBZRCQ	GTTCCTCACTCCTATTTCATCCA	R			51.7	742–764

Nested endpoint PCR and real-time RT-PCR primers designed. E: external; I: internal; F: forward; R: reverse. According to RABV strain SAD VA1 sequence.