Research Article
Development of Primer Pairs from Molecular Typing of Rabies Virus Variants Present in Mexico
Table 4
Comparison between different methods of rabies virus variants detection.
| | Sample | Host | FAT | Antigenic variant | External RT-PCR | Internal PCR | SYBR Green | GenBank sequence
AC number (N gene) | | D | V | B | S | d | v | b | s | d | v | b | s |
| | 1 | Dog 0068 | + | V1 | | | | | | | | | | | | | JQ037820 | | 2 | Dog 647 | + | V1 | | | | | | | | | | | | | JQ037819 | | 3 | Dog 659 | + | V1 | | | | | | | | | | | | | JQ037823 | | 4 | Dog 748 | + | V1 | | | | | | | | | | | | | JQ037821 | | 5 | Dog 2293 | + | V1 | | | | | | | | | | | | | JQ037824 | | 6 | Dog 885 | + | V1 | | | | | | | | | | | | | JQ037822 | | 7 | Cow 658 | + | V8 | | | | | | | | | | | | | JQ037825 | | 8 | Cow 2688 | + | V8 | | | | | | | | | | | | | JQ037826 | | 9 | Bat nonhematophagous 757 | + | A | | | | | | | | | | | | | JQ037830 | | 10 | Bat nonhematophagous 1594 | + | V5 | | | | | | | | | | | | | JQ037829 | | 11 | Bat nonhematophagous 1079 | + | None | | | | | | | | | | | | | JQ037831 | | 12 | Vampire bat 3919 | + | None | | | | | | | | | | | | | JQ037827 | | 13 | Vampire bat 110 | + | Atypical | | | | | | | | | | | | | JQ037828 | | 14 | Skunk 1369 | + | V8 | | | | | | | | | | | | | JQ037818 | | 15 | 65EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 16 | 543EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 17 | 642EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 18 | 223EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 19 | 1001EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 20 | 455EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 21 | 755EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 22 | 2187EDOMEXDOG05 | | NA | | | | | | | | | | | | | — | | 23 | Negative control | | NA | | | | | | | | | | | | | — |
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FAT: fluorescent antibody test; Varian antigenic test: V1, dog; V5, Tadarida brasiliensis; V8, skunk; V11, vampire bat; A, atypical; positive diagnosis. Capital letter: positive external RT-PCR; lowercased letter: positive internal PCR; italic letter: positive SYBR Green. Variants were represented in the following sense: D, dog; V, vampire; B, bat; and S, skunk. Sequences refer to NCBI accession number (http://www.ncbi.nlm.nih.gov/).
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