Identification of ITGA2B and ITGB3 Single-Nucleotide Polymorphisms and Their Influences on the Platelet Function

Table 2

Primer sequences used in this study.

DNA sequence number	Amplified or sequenced region	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Amplified region NC_000017.11	Length (bp)
ITGA2B-1		TCCTCCTCTTCCGCTTACCG	TACTACCACCGTGCTAGTCC	44389728–44390630	848
ITGA2B-2	Exon1	CCAATATGGCTGGTTGAG	AACTTCCCTTACGGCTCA	44389267–44390059	792
ITGA2B-3	Exon1	CCAGTGCAGCTCACCTTCTA	GATGAGGGAAATGGAACAGA	44388253–44389368	1115
ITGA2B-4	Intron1	TATGAACCACTCCACCCT	TTGGCACTCTTGATTCTG	44388169–44389006	837
ITGA2B-5	Exon2, Exon3	ACCGCTGGTTCTTGTTGC	CCTACGGGCGTCTTCTCA	44385649-44386479	830
ITGA2B-6	Exon4–Exon6	TACAGGGCACAGGGAACAATC	AGAGGCTCTGGGAGGACACG	44384990–44385810	820
ITGA2B-7	Exon7, Exon8	TCCTGGCGGCTATTATTTCT	GCACCGACGACATATTCTGG	44384339–44385186	847
ITGA2B-8	Exon9–Exon11	ATTTGCGCCCTTGTCCTC	AGCCGAATCGCCCATAGA	44383538–44384605	1067
ITGA2B-9	Exon12	CCCTCTGTCTCCCTTTCC	CATCCAGTCTCCCACCAA	44383189–44383838	649
ITGA2B-10	Exon13, Exon14	CCTAGTCTCCTGGGATGTTC	TCACGGGTGTCTTGGTCT	44380390–44381163	773
ITGA2B-11	Exon14–Exon17	TAATCGCCAATTCTGACCC	CACATCCCACCTTCTCCTG	44379854–44380682	828
ITGA2B-12	Exon18	ACCCACTGGACTTGTTCATC	TGTGACTTGGCACTAACCC	44379610–44379957	347
ITGA2B-13	Exon19, Exon20	TGGACGACAGAGCGAGAC	GGCCATACCTCGACATTG	44378354–44378989	635
ITGA2B-14	Exon21	CATGTGACAGTCCCTTGA	AAAGTCACTCACCCAAGGA	44377551–44377933	382
ITGA2B-15	Exon22	CTTGGAGGGTGAAGACTGG	CAACTCCTGACCTCCAGTGA	44376833–44377179	346
ITGA2B-16	Exon23–Exon25	CCAGGTCTAACTTCAGTGTG	GCTCTGGCAGGAAGATCTGT	44375629–44376523	894
ITGA2B-17	Exon26	TCCGACCTGCTCTACATCC	CGGGCTTGCTCACATAGTC	44375277–44375913	636
ITGA2B-18	Exon27, Exon28	ATGACCCTCCCTGCATCTC	CACCTTGACACCTGCCTTT	44374500–44375317	817
ITGA2B-19	Exon29	GCACGCATGGTTCAACGT	CCTCCCGAGTAGCTGAGATT	44374025–44374731	706
ITGA2B-20	Exon30	AAAGGCATCCATTTGTGA	TGTTGGTAAGGCTGGTCTC	44371896–44372566	670
ITGB3-1		CAGGAGGTGGAGGATTGT	GCTGGATTCTTGGGACAC	47252637–47253474	837
ITGB3-2	Exon1	CGGTTCAGAGAAGGCATTCAG	GCTCCAAGTCCGCAACTTGA	47253373–47254015	642
ITGB3-3	Intron1	TTGGCGTAGGAGGTGAGTGA	CCGCAGGAAGCCAAGTTGAA	47253929–47254518	589
ITGB3-4	Intron1	TTGGCGTAGGAGGTGAGTGAG	GAAGTTGCAGTGAGCCGAGAA	47253929–47255063	1134
ITGB3-5	Exon2	ATTGGGAAAGTTGGGAAGG	GAAAGGGCAGCAGTGGTT	47274335–47274773	438
ITGB3-6	Exon3	AGGCTGGTCTTGAACTCTTG	CTCCACCTTGTGCTCTAT	47283116–47283612	496
ITGB3-7	Exon4	GGGCTTTCTGGTTTGCTT	CATTTCCCTCCCATTCTC	47284329–47284978	649
ITGB3-8	Exon5	TGTCTGGGTAACTGTGGT	CATCTGCCTACTTTGCTG	47286124–47286725	601
ITGB3-9	Exon6	TCCAAGGACTGGGACTGA	ATGATGCTGCTGCTATGC	47286919–47287483	564
ITGB3-10	Exon7, Exon8	AGCCCAAGCAAGATAAGT	GGAGAAGGCAGTAAGACC	47289597–47290393	796
ITGB3-11	Exon9	AAACTGGGCTCCAATAAC	TGAGGGACTGAAGGTAAAG	47290561–47291406	845
ITGB3-12	Exon10	CAGGGCAGGGAACAACTT	GGATTGGTCCTTATACTCAAAA	47292050–47292720	670
ITGB3-13	Exon11	GAGCAAGTCCTGCCATAC	TCACAGAGTGTCCTCCATAA	47299101–47299890	789
ITGB3-14	Exon12	CAGAAATGGCATAGGGTT	TCTTGCTGAGTCTGTGGG	47300168–47300824	656
ITGB3-15	Exon13	CTTGAATCTAGGCATCGT	GTATTGAACTCCTGACCC	47302581–47303050	469
ITGB3-16	Exon14	CCTCAAGTAGGTCCCAGTG	AACATGACCACCCAAAGC	47307158–47307721	563
ITGB3-17	Exon15	CTCATCTCCTCCTGTTATTT	TGACATTCTCCCAACCTAC	47309941–47310337	396