KvLEA, a New Isolated Late Embryogenesis Abundant Protein Gene from Kosteletzkya virginica Responding to Multiabiotic Stresses

Figure 1

Sequence, hydrophilic, repeat motifs and phylogenetic analysis of KvLEA. (a) The nucleotide and predicted amino acid sequences of KvLEA gene. The initiation codon and termination codon were highlighted with red underline. The encoded amino acids were listed correspondingly to the ORF. (b) Hydrophilic analysis of KvLEA. The analysis was conducted according to Kyte-Doolittle hydropathy plot. The section above zero stands for the hydrophobic part of the sequence and the section below zero represents the hydrophilic part. (c) The BLASTp search of the predicted amino acid sequence. The red boxes show the category and position of the conserved motifs (LEA_4 accession: pfam02987). (d) Phylogenetic tree of KvLEA and eighteen LEA_4 proteins in Arabidopsis. The phylogenetic tree was constructed using MEGA 6.0 by Neighbor-Joining method based on the multiple sequence alignment of the nineteen LEA proteins. The protein sequences of the Arabidopsis LEA genes were acquired from the Arabidopsis Information Resource (TAIR) database (http://www.arabidopsis.org/). The gene IDs and names of the proteins were listed in Supplemental S3.

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