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BioMed Research International
Volume 2017, Article ID 4176170, 11 pages
Research Article

The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse

1Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, China
2Department of Clinical Medicine, Zhejiang University City College School of Medicine, Hangzhou, China
3Department of Thoracic Surgery, Zhejiang Cancer Hospital, Hangzhou, China
4Department of Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, China

Correspondence should be addressed to Jing-yin Dong; nc.ude.ccuz@yjgnod and Qiu Yan; moc.621@36uiqnay

Received 23 December 2016; Revised 20 March 2017; Accepted 11 April 2017; Published 21 May 2017

Academic Editor: Janusz Blasiak

Copyright © 2017 Wei Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ionizing radiation may cause irreversible ovarian failure, which, therefore, calls for an effective radioprotective reagent. The aim of the present study was to evaluate the potential radioprotective effect of N-acetylcysteine (NAC) on ionizing radiation induced ovarian failure and loss of ovarian reserve in mice. Kun-Ming mice were either exposed to X-irradiation (4 Gy), once, and/or treated with NAC (300 mg/kg), once daily for 7 days before X-irradiation. We examined the serum circulating hormone levels and the development of ovarian follicles as well as apoptosis, cell proliferation, and oxidative stress 24 hours after X-irradiation. In addition, morphological observations on the endometrial luminal epithelium and the fertility assessment were performed. We found that NAC successfully restored the ovarian and uterine function, enhanced the embryo implantation, improved the follicle development, and altered the abnormal hormone levels through reducing the oxidative stress and apoptosis level in granulosa cells while promoting the proliferation of granulosa cells. In conclusion, the radioprotective effect of NAC on mice ovary from X-irradiation was assessed, and our results suggested that NAC can be a potential radioprotector which is capable of preventing the ovarian failure occurrence and restoring the ovarian reserve.