The Protective Effect of N-Acetylcysteine on Ionizing Radiation Induced Ovarian Failure and Loss of Ovarian Reserve in Female Mouse

Figure 5

Oxidative stress analysis. (A) Immunohistochemical localization of 8-OHdG in ovary tissue. Expression of 8-OHdG showed a minimum degree in control (a) and NAC treatment group (c), while, in X-irradiation group (b), the 8-OHdG formation was highly increased, and pretreatment of NAC limited the 8-OHdG expression (d). Scale bar, 20 μm. (B) Quantitative image analysis for IHC staining expressed as mean density in each group. Each column represents the mean ± SD of at least three independent experiments ( versus control; versus X-irradiation group). (C) GPx activity level in wet ovary tissue was represented as mU/mg of protein. X-irradiation significantly decreased the GPx activity concentration. Each column represents the mean ± SD of at least three independent experiments. ( versus control; . versus X-irradiation group).