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BioMed Research International
Volume 2017, Article ID 5158961, 10 pages
Research Article

Substrate Stiffness Influences Doxorubicin-Induced p53 Activation via ROCK2 Expression

1Frontiers of Innovative Research in Science and Technology, Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan
2Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan
3Laboratory for Comprehensive Bioimaging, Riken Qbic, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
4WPI, Immunology Frontier Research Center, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
5Laboratory for Mechanical Medicine, Nadogaya Research Institute, Nadogaya Hospital, 687-4 Kashiwa, Chiba 277-0032, Japan
6Department of Rehabilitation for the Movement Functions, Research Institute, National Rehabilitation Center for Persons with Disabilities 4-1 Namiki, Tokorozawa, Saitama 359-8555, Japan
7Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, Aichi 466-8550, Japan
8Department of Molecular Oncology, Institute for Advanced Medical Sciences, Nippon Medical School, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan

Correspondence should be addressed to Keiko Kawauchi;

Received 30 July 2016; Revised 3 December 2016; Accepted 12 December 2016; Published 16 January 2017

Academic Editor: Esmaiel Jabbari

Copyright © 2017 Takahiro Ebata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Fig. S1. Doxorubicin decreases the viability of cells cultured on substrates with an elasticity of 30 kPa compared to those with an elasticity of 2 kPa in a p53-dependent manner. Fig. S2. Soft substrates increase the amount of p53 in the Triton-X insoluble fraction.

  1. Supplementary Material