Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H₂O₂-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells

Figure 3

Low-concentration H₂O₂ induced ERK5 activation in HUVECs. (a) ERK5, p-ERK5, MEF2C, p-MEF2C, ERK1/2, and p-ERK1/2 protein levels were measured by western blotting. Following treatment with PBS, H₂O₂ (50 μmol/L), or catalase (5 U/ml) and H₂O₂ (50 μmol/L) for 30 min, cells were lysed, and the lysates were resolved by 10% or 6% SDS-PAGE. (b, c, d, e, f, g) Densitometric quantification of ERK5, p-ERK5, MEF2C, p-MEF2C, ERK1/2, and p-ERK1/2 expression (mean ± SD of three separate experiments, versus control, and one-way ANOVA followed by SNK post hoc test).

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