Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions
Table 2
Relative contribution of experimental sequences to RNA classes in human blood cells (I), plasma (II), and fractions of blood plasma (III–V) of healthy donors and lung cancer patients. For each experimental read, a single position in the references was allowed.
Fractions(1)
(I) Blood cells
(II) Plasma
(III) 16,000 pellets
(IV) 160,000 pellets
(V) 160,000 supernatant
Donors or patients(2)
H
LSCC
LAC
H
LSCC
LAC
H
LSCC
LAC
H
LSCC
LAC
H
LSCC
LAC
Relative contribution (%)
Mitochondrial RNAs
2.45
5.38
0.72
13.76
8.89
0.33
17.84
12.01
1.01
2.83
1.16
0.12
7.52
0.61
0.09
rRNA
23.69
18.21
27.63
35.01
18.06
4.36
40.39
19.89
1.81
20.60
25.17
51.27
29.61
12.18
2.20
tRNA
0.70
0.36
2.74
2.39
0.70
0.21
0.42
0.23
1.05
0.50
0.57
0.52
1.06
0.86
0.53
YRNA
1.83
0.88
1.53
1.48
1.31
0.37
5.01
1.29
4.80
1.31
0.79
0.64
1.62
0.25
3.73
U1–U17 snRNA and 7SK RNA
0.52
0.22
0.56
0.20
0.07
0.07
0.06
0.04
0.02
0.17
0.31
0.70
0.18
0.15
0.12
SINE(3)
1.18
0.27
0.79
0.61
0.28
0.16
0.64
0.47
0.08
0.17
0.26
0.14
0.51
0.13
0.06
LINE(3)
0.46
0.23
0.17
0.23
0.35
0.15
0.15
0.28
0.14
0.18
0.32
0.17
0.28
0.51
0.18
LTR(3)
0.09
0.06
0.04
0.06
0.09
0.02
0.04
0.07
0.05
0.03
0.10
0.08
0.07
0.17
0.05
DNA(3)
0.04
0.08
0.17
0.03
0.05
0.02
0.02
0.05
0.04
0.08
0.06
0.08
0.05
0.06
0.03
Other transcribed repeats(4)
0.03
0.07
0.03
0.09
0.07
0.02
0.06
0.05
0.03
0.05
0.25
0.13
0.75
3.85
0.03
mRNA(5)
22.50
30.54
26.08
19.80
34.12
30.24
15.21
29.88
31.28
25.16
33.98
20.11
21.09
35.80
31.36
ncRNA(6)
22.71
3.55
2.95
2.84
2.92
4.16
1.62
3.27
16.30
2.25
3.13
2.95
3.60
3.19
17.93
Non-RefSeq RNA(7)
23.79
40.14
36.60
23.50
33.10
59.89
18.54
32.47
43.39
46.67
33.89
23.08
33.67
42.23
43.69
Number of reads(8)
Total(8)
Blood fractions according to Table 1. Pooled blood samples of healthy donors (H), patients with lung squamous cell carcinoma (LSCC), and patients with lung adenocarcinoma (LAC). Reads mapped on referenced sequences of SINE-, LINE-, LTR-, and DNA-repeat families from GIRI Repbase. SINE also includes sequences aligned to 7SL RNA (NR_002715). Includes satellites and simple and other repeats from GIRI Repbase. Fragments mapped to mature mRNAs referenced as exons of NM records of NCBI human RefSeq RNA. Fragments of exons of NR records of human RefSeq RNA. Reads mapped to human genome sequences include introns of NM/NR human RefSeq RNAs as well as fragments of novel lncRNAs not annotated in the human RefSeq RNA database. Total number of aligned reads.
