Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent

Table 2

The effects of different metal ions on enzyme activity.

Metal ion	Relative activity (%)
	1 mM	10 mM
Control	100±2.5	100±2.5
K^+	105.8±5.7	121.3±4.0
Ca^2+	121.3±2.9	79.1±3.9
Mg^2+	115.1±3.6	171.2±2.5
Al^3+	88.8±3.7	76.9±3.7
Fe^2+	82.5±2.7	68.3±2.4
Fe^3+	98.4±2.1	87.9±4.4
Mn^2+	56.2±2.2	33.7±4.7
Zn^2+	91.2±2.1	101.4±4.4
Cu^2+	46.6±3.4	24.1±2.1
Hg^2+	44.9±6.7	36.9±4.3
Pb^2+	112.3±2.0	73.1±4.1
Ba^2+	119.4±3.0	78.9±6.5
Ni^2+	139.4±2.9	150.2±3.1
Cd^2+	71.3±2.8	56.4±4.9