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BioMed Research International
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2018
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Article
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Tab 3
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Research Article
Molecular Cloning and Characterization of a Novel
α
-Amylase from Antarctic Sea Ice Bacterium
Pseudoalteromonas
sp. M175 and Its Primary Application in Detergent
Table 3
The effects of chemical reagents on enzyme activity.
Chemical reagents
Concentration
Relative activity (%)
Control
None
100±4.5
Tween 80
1%
50.8±3.8
10%
48.4±6.3
Triton X-100
1%
79.1±4.2
10%
69.9±4.1
DMSO
1%
95.7±4.3
10%
141.9±5.4
SDS
1 mM
118.8±4.6
10 mM
77.2±3.8
EDTA
1 mM
102.0±5.1
10 mM
88.9±4.0
DTT
1 mM
84.9±5.1
10 mM
74.7±4.0
β
-ME
1 mM
75.8±5.1
10 mM
31.9±4.7
Urea
1 mM
98.9±5.7
10 mM
86.9±4.0