Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent

Table 3

The effects of chemical reagents on enzyme activity.

Chemical reagents	Concentration	Relative activity (%)
Control	None	100±4.5
Tween 80	1%	50.8±3.8
	10%	48.4±6.3
Triton X-100	1%	79.1±4.2
	10%	69.9±4.1
DMSO	1%	95.7±4.3
	10%	141.9±5.4
SDS	1 mM	118.8±4.6
	10 mM	77.2±3.8
EDTA	1 mM	102.0±5.1
	10 mM	88.9±4.0
DTT	1 mM	84.9±5.1
	10 mM	74.7±4.0
β-ME	1 mM	75.8±5.1
	10 mM	31.9±4.7
Urea	1 mM	98.9±5.7
	10 mM	86.9±4.0