Development of Two Analytical Methods Based on Reverse Phase Chromatographic and SDS-PAGE Gel for Assessment of Deglycosylation Yield in -Glycan Mapping

Table 1

Evaluation of enzymatic deglycosylation kinetics in fetuin processed with reagents and protocol from GlycanAssure HyPerformance APTS kit.

Sample lane # on the gel in Figure 1	2	3	4	5	6	7
Time of deglycosylation (min)	0	1	3	5	8	10
Glycosylated peak (raw )	3422	1912	686	275	0	0
Deglycosylated peak (raw )	0	2860	3560	3249	3482	3906
Deglycosylation (%)	0	60	84	92	100	100

volume refers to density of each band from SDS-PAGE gel image representing the amount of glycosylated and deglycosylated proteins.