Reperfusion Activates AP-1 and Heat Shock Response in Donor Kidney Parenchyma after Warm Ischemia

Figure 2

Highly connected networks built using reperfusion-upregulated mRNAs coding for proteins known to interact with human proteins. (a) Protein-Protein Interaction Network (PPIN) generated using the intersection of mRNAs sets unidirectionally overexpressed after reperfusion in Kidney 1 and Kidney 2. (b) PPIN generated using set of mRNAs detected to be overexpressed after comparing combined amounts of reads from both kidneys before and after reperfusion. Network was generated by Pathway Studio Network Building. Each node represents a protein. Network hubs are highlighted in blue (Jun/Fos/ATF hub) or green (HSP1A/ HSPA5 hub). In Figure 2(a), stars indicate the targets of transcription factors overexpressed in reperfusion. In Figure 2(b), protein ITGB3 is highlighted in yellow as the only entity added to the network as a result of read combining.

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