Multiplex Real-Time Polymerase Chain Reaction for Simultaneous Quantification of Salmonella spp., Escherichia coli, and Staphylococcus aureus in Different Food Matrices: Advantages and Disadvantages

Table 3

Specific gene copy numbers in one colony-forming unit (cfu) through TaqMan qPCR.

Microorganism (target gene)	Ct	SD	CV (%)	Gene copy number/cfu
Salmonella (ssf)	58.78	0.20	0.34	2.10 × 10^8
Escherichia coli (phoA)	46.94	0.28	0.60	1.28 × 10^7
Staphylococcus aureus (nuc)	47.48	0.64	1.37	7.9 × 10^11

Average between three replicates.
Ct: cycle threshold; SD: standard deviation; CV: coefficient of variation.