Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater

Table 2

Experimental design of steepest ascent and corresponding results.

Run	Loaded liquid (mL/250mL)	pH	Culture temperature (°C)	Total number of living B. megaterium (10^7Obj/mL)
1	150	5.5	40	0.17 ± 0.01
2	120	6.0	36	0.19 ± 0.01
3	90	6.5	32	2.96 ± 0.02
4	60	7.0	28	4.79 ± 0.02
5	30	7.5	24	14.91 ± 0.03
6	15	8.0	20	0.11 ± 0.01

Data are expressed as the mean ± standard deviation (SD) of 3-batch independent experiments.