Research Article
Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater
Table 3
Experimental design and results of Box-Behnken design.
| | Run | X1: Loaded liquid (mL/250mL) | X2: pH | X3: Culture temperature (°C) | Total number of living B. megaterium (107Obj/mL) | | Code level | Real level | Code level | Real level | Code level | Real level |
| | 1 | 0 | 30 | 0 | 7.5 | 0 | 28 | 7.91 ± 0.02 | | 2 | 0 | 30 | 0 | 7.5 | 0 | 28 | 8.67 ± 0.03 | | 3 | 0 | 30 | -1 | 6.5 | -1 | 24 | 1.02 ± 0.02 | | 4 | 0 | 30 | 0 | 7.5 | 0 | 28 | 8.50 ± 0.02 | | 5 | 1 | 60 | 0 | 7.5 | 1 | 32 | 3.41 ± 0.02 | | 6 | 1 | 60 | 1 | 8.0 | 0 | 28 | 1.12 ± 0.01 | | 7 | -1 | 15 | -1 | 6.5 | 0 | 28 | 4.03 ± 0.02 | | 8 | -1 | 15 | 0 | 7.5 | 1 | 32 | 5.41 ± 0.03 | | 9 | -1 | 15 | 1 | 8.0 | 0 | 28 | 6.04 ± 0.03 | | 10 | 1 | 60 | 0 | 7.5 | -1 | 24 | 0.25 ± 0.02 | | 11 | 0 | 30 | 0 | 7.5 | 0 | 28 | 8.57 ± 0.03 | | 12 | 0 | 30 | 0 | 7.5 | 0 | 28 | 8.75 ± 0.04 | | 13 | 0 | 30 | 1 | 8.0 | 1 | 32 | 4.62 ± 0.02 | | 14 | 0 | 30 | 1 | 8.0 | -1 | 24 | 1.63 ± 0.01 | | 15 | 1 | 60 | -1 | 6.5 | 0 | 28 | 2.04 ± 0.02 | | 16 | -1 | 15 | 0 | 7.5 | -1 | 24 | 2.62 ± 0.01 | | 17 | 0 | 30 | -1 | 6.5 | 1 | 32 | 4.40 ± 0.02 |
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Data are expressed as the mean ± standard deviation (SD) of 3-batch independent experiments.
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