Systemic Infection of<i> Nicotiana benthamiana</i> with<i> Potato virus X</i> Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

<div>Detection of green fluorescence in epidermal cells from<i> N. benthamiana</i> leaves systemically infected with pPVX-iLOV-CP and pPVX-iLOV-2A-CP. The iLOV fluorescence was observed by confocal laser scanning microscopy. The hybrid particles indicate cell-to-cell movement at plasmodesmata (PD) and viral replication complexes (VRCs). For iLOV-2A-CP there is also free iLOV visible in the cytoplasm (<svg height="7.34169pt" id="M8" style="vertical-align:-0.3499303pt" version="1.1" viewbox="-0.0498162 -6.99176 7.75925 7.34169" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M535 253V257L73 504L71 502V9L73 7L535 253Z" id="g114-159"></path></g></svg>). N: nucleus.</div>

BioMed Research International

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Figure 4

Figure 4: Systemic Infection of<i> Nicotiana benthamiana</i> with<i> Potato virus X</i> Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein 

Figure 4 | Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein 