Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

Figure 5

Analyses of purified iLOV-CP and iLOV-2A-CP particles. (a–c) Purified PVX particles (5 μg) displaying iLOV separated by SDS-PAGE. M: P7711 Prestained Protein Standard (NEB), 1: iLOV-CP sample 1, 2: iLOV-CP sample 2, 3: iLOV-CP sample 3, 4: iLOV-2A-CP sample 1, 5: iLOV-2A-CP sample 2, 6: iLOV-2A-CP sample 3, 7: negative control, noninfected N. benthamiana leaf extract, 8: PVX-GFP-2A-CP infected leaf extract, and 9: positive control, PVX particles. Antibody detection: (b) anti-PVX and and (c) anti-iLOV and . (d–e) Transmission electron microscopy of recombinant particles. Adsorption: detection with anti-iLOV and decoration with GAR 15 nm gold conjugate, ISEM: capture with monoclonal MAC58, detection with anti-iLOV, and decoration with GAR 15 nm gold conjugate. Bar = 500 nm.