BioMed Research International

BioMed Research International / 2019 / Article

Research Article | Open Access

Volume 2019 |Article ID 2080352 | https://doi.org/10.1155/2019/2080352

Achraf Laghmich, Fatima Zahra Alaoui Ismaili, Amina Barakat, Naima Ghailani Nourouti, Mohamed Khattab, Mohcine Bennani Mechita, "Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum", BioMed Research International, vol. 2019, Article ID 2080352, 7 pages, 2019. https://doi.org/10.1155/2019/2080352

Alpha-Thalassemia in North Morocco: Prevalence and Molecular Spectrum

Academic Editor: Pedro Cabrales
Received14 Oct 2018
Revised09 Jan 2019
Accepted24 Feb 2019
Published13 Mar 2019

Abstract

Unlike the other hemoglobinopathies, few researches have been published concerning α-thalassemia in Morocco. The epidemiological features and the mutation spectrum of this disease are still unknown. This regional newborn screening is the first to study α-thalassemia in the north of Morocco. During the period from January 2015 to December 2016, 1658 newborns umbilical blood samples were investigated. Suspected newborns were screened for α-globin defects using Gap-PCR and Multiplex Ligation-dependent Probe Amplification technique. The prevalence of α-thalassemia, its mutation spectrum, and its allelic frequencies were described for the first time in Morocco. Six different α-globin genetic disorders were detected in 16 neonates. This screening valued the prevalence of α-thalassemia in the studied population at 0.96% and showed the wide mutation spectrum and the heterogeneous geographical distribution of the disease. A high rate of carriers was observed in Laouamra, a rural commune in Larache province. Heterogeneity of α-globin alleles in Morocco explains the high variability of α-thalassemia severity. This diversity reflects the anthropological history of the country. These results would contribute to the prevention of thalassemia in Morocco directing the design of a nationwide screening strategy and awareness campaign.

1. Introduction

α-Thalassemia represents a group of recessively inherited hemoglobin disorders due to deficient or absent synthesis of α-globin genes. Affecting 5% of the world’s population, α-thalassemia is the most common monogenic disorder [1]. The clinical phenotype of α-thalassemia varies according to the number of affected genes from almost asymptomatic to a lethal hemolytic anemia. More than 80 different genetic defects have been described; most of them are deletions and less commonly nondeletional defects involving α-globin genes [2, 3]. This leads to the wide spectrum of hematological and clinical phenotypes. Genotype-phenotype correlation is still not well documented [4].

α-Thalassemia is prevalent in populations of Southeast Asia, Central Africa, and the Middle East [5, 6]. In the last few decades, massive population migrations favored the spread of these defects. Some abnormal Hb variants, once specific to particular ethnic groups, are now worldwide spread [7, 8]. Morocco, a Mediterranean country, is a potential predilection zone of hemoglobinopathies due to its geographical position. The frequency of the α-mutated genes is believed to be increased due to the country’s socioeconomic and cultural system encouraging consanguinity, the selective pressure of malaria in endemic close countries, and its history as a migration crossroad between sub-Saharan Africa and Europe [9, 10]. The World Health Organization estimates the rate of hemoglobinopathies carriers in Morocco at 6.5%, with more than 30 000 cases of major forms [11]. A previous regional study highlighted the region of North Morocco as a geographical hot-spot for hemoglobinopathies due to its high consanguinity rate already estimated and its location in the malaria belt [10, 12]. Nevertheless, very scarce data about the occurrence and epidemiology of α-thalassemia is available. Thus, the overarching aim of our present study is assessing the prevalence and spectrum of α-thalassemia in the north of Morocco. Our conclusions would help decoding the context of the disease epidemiology and lead to a better formulation of country-specific and optimized α-thalassemia policies.

2. Population and Methods

A total of 1658 newborns were screened for α-thalassemia in the three provincial hospitals of Tangier, Tetouan, and Larache cities between January 2015 and December 2016. The studied region is located in the northwestern part of Morocco and on the southern Mediterranean coast of the thalassemic belt (Area: 7098.8 km2, Population: 2386852 inhabitants (national census of 1st September 2014) [13]). The choice was based on the region’s history with malaria, its high consanguinity, and the increase in length of stay and cumulative hospitalized days due to hemoglobinopathies in the region hospitals. Figure 1 shows the geographic location of the studied region divided into three provinces: Tangier Assilah Fahs-Anjra, Tetouan M’diq-Fnideq, and Larache (Figure 1).

Our study is a newborn screening, using new techniques of molecular biology, for an accurate determination of the α-thalassemic genotypes and their frequencies. This can lead diagnostic centers to develop an effective genetic counseling of couples and a better diagnosis.

Umbilical blood samples of 4ml volume were taken by qualified midwives immediately after delivery on EDTA tubes from newborns in the hospitals. Each sample was accompanied by prior consent signed or thumb printed by the mothers. All the parents were of the region descents and were well informed about the study procedures and objectives. Information sheets with nationality, sex, age, dialect, and natives or not and written consent forms were available in Arabic to ensure comprehensive understanding of the study objectives. This study was approved by the Ethics Committee for Biomedical Research in the Faculty of Medicine and Pharmacy of Rabat (CERB), Morocco.

Several Researches have studied the appropriate cutoff values of hematologic features for α-thalassemia. In adults, α-thalassemia carriers’ diagnosis is based on microcytosis and normal HbA2 level [14]. In newborns, an effective primary screening with 100% accuracy could be carried out using mean corpuscular volume less than 95fL, mean corpuscular hemoglobin less than 30 pg, and/or the presence of hemoglobin Bart’s fraction [15, 16]. These were the inclusion criteria in our study.

All the newborns were analyzed for hematological indices, including red blood cells (RBC), hemoglobin concentration (Hb), mean cell volume (MCV), mean cell hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) using an automated cell counter SYSMEX XT-1800 (Kobe, Japan). Ferritin level was assessed with established protocols using the automated VIDAS-BIOMERIEUX Immunoanalyzer, and the Hb separation studies were done by capillary electrophoresis method (MINICAP Sebia, Paris, France).

Genomic DNA was isolated from leukocytes using the QIAamp® Blood Mini Kit (Qiagen, Germany) and stored at − 20°C. Molecular screening of most common α-globin mutations (-α3.7, -α4.2) was performed using Gap-PCR (Gap-Polymerase Chain Reaction) as previously described by Dodé and Samuel S. Chong [17]. MLPA analysis was carried out using the SALSA MLPA Kit, HBA140-B4 Kit (MRC- Holland, Netherlands), according to the manufacturer’s instructions [18]. It was used to detect copy number changes of 26 different sequences in the α-globin cluster on chromosome 16p13.3. PCR was performed with a standard thermocycler (Eppendorf, Germany), and the separation of the amplification products was done by capillary electrophoresis on the Genetic Analyser 3130XL (Applied Biosystems®). Then the results were analyzed with Coffalyser® (Applied Biosystems®). The deletional origin of α-thalassemia was investigated as no point mutation was detected in the studied region during routine diagnosis tests. Deletions are known to be responsible for more than 95% of α-thalassemia defects in the world [19]. This was performed in Biochemistry and Molecular genetics laboratory at Cadiz University, Spain.

Our data were analyzed using SPSS version 11.5 statistical software. An independent-sample test was carried out to compare the different hematological parameters of normal subjects and those suspected of being α-thalassemic. Statistical significance was assessed as a test with p ≤ 0.05.

3. Results

Our study estimates the prevalence of α-thalassemia, its mutations spectrum, allelic frequencies, and its geographical distribution in the north of Morocco. The hematological analysis revealed 26.59% of anemic newborns (441/1658). The Complete Blood Count profile was similar in the three provinces with a mild increase of hypochromic anemia in Larache province (Table 1).


ProvincepopulationAnemicNon AnemicMicrocytic hypochromic anemiaOther anemiaFerritin level
LowNormalHigh

Tangier, Assilah-Fahs-Anjra6331634707984521116

Tetouan, M’diq, Fnideq483109374664346515

Larache5421693731195075935

means other anemia: macrocytic normochromic, macrocytic hypochromic, normocytic normochromic, and normocytic hypochromic.

Microcytosis was always accompanied by Hypochromia and it was found in 264 newborns. Hypochromic nonmicrocytic anemia was assessed at 67 newborns. The assessed iron status revealed a heterogeneity in the distribution of ID in the three studied provinces. Significant increase of low ferritin was observed in Tetouan M’diq-Fnideq province, while newborns from Larache province registered significant higher levels of cord blood ferritin. The ID was found in 173 newborns in the studied region (10.43%).

Capillary electrophoresis of hemoglobin was assessed to quantify the different Hb variants in the newborns. No newborn has shown Hb H, Hb C, Hb E, or Hb D variants. Hb Bart’s was identified in 17 newborns, 2 in Tangier Assilah Fahs-Anjra province, 2 in Tetouan M’diq-Fnideq province, and 13 in Larache province. The percentage of Hb Bart’s carriers at birth was estimated at 1.02% (17/1658). The highest frequency was registered in Larache province with 2.39% (13/542).

Gap-PCR technique detected the presence of 11 α-thalassemic cases, resulting from two different molecular defects: -α3.7 deletion and –α4.2 deletion. The results of MLPA confirmed the cases diagnosed by Gap-PCR and revealed new α-thalassemic cases with novel mutations detected for the first time in the Moroccan population. Six different molecular defects on the-α globin gene are detected defining 8 genotypes (Table 2). MLPA results also showed a newborn with the African polymorphism α2. This polymorphism, detected for the first time in the Moroccan population, does not lead to thalassemia (Tables 2 and 3 & Figure 2). All the detected newborns carrying α-thalassemic defects were anemic at birth.


Molecular defectAllele frequencies (%)GenotypesN

-α3.70.33-α3.7/ αα6
- / αα1
-α3.7/ -α3.72

-α4.20.12-α4.2/ αα2
-α4.2/ -α4.21

-αα0.06-αα/ αα2

HS-400.03αα/ αα with HS-40 del1

--Med I0.03--/αα1


GenotypeProvinceHb  
(g/dl)
MCV  
(fl)
MCH  
(pg)
Hb Bart’s  
%
Tangier Assilah Fahs-AnjraTetouan M’diq FnideqLarache

-α3.7/ αα21311.5126.235.40.8
13.192.629.20
13.594.729.91.8
12.592.528.70.45
15.593.529.60.9
12.59433.80

-α3 .7/-α3.700211.195.929.91.8
13.398.531.52.3

- /αα00111.896.330.31.3

αα/αα10111.293.328.50
11.493.728.90

-α4.2/ αα00211.995.628.30.4
13.29131.91

-α4.2/ -α4.201012.792.729.93

αα/ αα with HS-40 del00111.292.429.81.1

--/αα00112.188.529.55.2

Hb: hemoglobin, MCV: mean corpuscular volume, MCH: mean corpuscular hemoglobin.

A heterogeneous geographical distribution of α-thalassemia in the studied region was revealed. The highest frequency was registered in Larache province 2.02%. However, Tangier Assilah Fahs-Anjra province and Tetouan M’diq-Fnideq province registered significantly low frequencies 4.73x10−3% and 4.14 x10−3%, respectively. α-Thalassemia prevalence was valued at 0.96% (16/1658) in the studied region. A preponderance of males was recorded with a frequency of 56.25% against 43.75% for females with a male to female sex ratio of 1.28.

-α20.5, --, --, --, -- deletions were not found in any studied newborn. Among the 16 newborns who had α-thalassemia molecular defects, only 12 carried Hb Bart’s at birth.

Comparison of the hematological and electrophoretic data of newborns with the same mutation and between newborns with different mutations has shown a significant difference in phenotype severity between the silent carries and the alpha-thalassemic newborns. -- deletion and HS-40 deletion were associated with the most severe phenotypes.

4. Discussion

Hematological studies showed that 26.59% of studied newborns were anemic; 59.86% of them had microcytic hypochromic anemia (MHA), considered as the hallmark feature of α-thalassemia [20]. To our knowledge, no neonatal anemia study was reported in Morocco. The obtained anemia rates are similar to other studies in adults in Morocco [21, 22]. Hb Bart’s carriers in the population were estimated at 1.02%. This rate is the lowest reported in Africa (7.38% in Tunisia [23], 10% in Algeria [24], 14.5% in Congo [25], and 11.5% in Tanzania [26]). Three quarter (12 neonates) of the newborns with Hb Bart’s at birth had α-thalassemia molecular defects. Our results are similar to those reported in the Tunisian population [23].The absence of Hb Bart’s in cord blood does not exclude the silent carrier state and a mild increase of Hb Bart’s fraction does not ascertain a loss of α-globin loci [27, 28]. Four diagnosed α-thalassemic newborns showed no Hb Bart’s fraction (Table 2). Five nonthalassemic newborns showed a fraction of Hb Bart’s (not microcytic not hypochromic). Four of them registered a fraction of 0.1% each and one showed a fraction of 0.3%. The cutoff was estimated already at 0.2% by Munkongdee T et al. [29]. The healthy newborn exceeding the already estimated cutoff was investigated for point mutations without positive result. In our samples the cutoff was estimated at 0.3%; more studies should be done to confirm this result.

α-Thalassemic male to female sex ratio was estimated at 1.28. This is in agreement with other studies carried out in other populations (Bangladesh 1.26 [30], India 1.14 [31], Iran 1.21 [32], Tunisia 1.24 [33], Lebanon 1.32 [34]). This variance is noteworthy and merits further study, as thalassemia is a single gene disease transmitted by a recessive mode on inheritance. No difference in the prevalence of thalassemia between both sexes has been reported [35, 36].

No hydrops fetalis syndrome was diagnosed during the study. The rare hemoglobin variants already described in the Moroccan, Tunisian, and Algerian population, Hb H, Hb C, Hb O-Arab, Hb D-Punjab, Hb Philadelphia, and Hb Lepore, were absent in the region [25, 26]. This absence is evidence for their low prevalence in the studied region. Unpublished archives in the medical centers describe some rare cases of Hb O-Arab and Hb C variants, and hence the need for an enlarged sample’s number screening for a precise determination of these variants.

Genetic analysis identified six different α-thalassemia defects, diagnosed for the first time in the Moroccan population. In Tunisia, nine mutations were described to be responsible for this disease [37], and six in the Algerian population [38]. The regional prevalence being estimated at 0.96% seems to be the lowest in the Mediterranean basin (Algeria 4.6%, Tunisia 4.8-5.4%, Libya 1-5%, Cyprus 10.6%) [39, 40]. Larache province was the most affected with a frequency of 2.02% (11/542). This disproportioned distribution between the three provinces could be explained by the different consanguinity rates, different history with malaria, and disparity in terms of the availability of primary care services and genetic counseling [11].

-α3.7 deletion is the most common mutation found in the North Moroccan population (allele frequency 0.33%). However, it is less frequent compared with the other Mediterranean countries (Algeria 2.9% [38], Tunisia 4.5%, Sicily 4.1% in 2002 [37], and Cyprus 7.7% in 2000 [39]). -α3.7 deletion was associated, in one newborn, with a heterogeneous deletion in cis on the ζ gene already detected in his father. To our knowledge, this association was never reported before in literature.

In our study -α4.2 allele was found at 0.12% in our samples. Higher frequencies were registered in Bahrein 2% [41], Tunisia 0.9% [37], and Malaysia 0.6% [42]. − was reported in our samples at 0.06%. It was estimated at 1% in the Cyprus population in 2000 [39]. HS-40 deletion allele and α0 --Med-I allele were found in lower frequencies (0.03% each). HS-40 deletion, already reported in the Brazilian population [43] but never in North Africa and Middle East [38, 44], is caused by the loss of the major regulatory element of the human alpha-globin locus, located 40 kb upstream of the zeta-globin gene, essential for α globin expression. -- MED-I, also responsible for α0-thalassaemia, is relatively frequent in Greece, Turkey, and the Middle East [3]. In Algeria this mutation was reported at 0.3% [38], Cyprus at 0.5% [39]. MLPA results showed also the existence of the African polymorphism with a frequency of 0.03%; this polymorphism is not related to any thalassemic phenotype. In Tunisia this polymorphism has an allele frequency esteemed at 0.99% [37].

-α3.7, -α4.2, and -- were already reported together in North Africa and Arab world [38, 45, 46]. This suggests that the origin of the common mutations spread is the same because of the ongoing genetic exchange all along this region over time. The possible introduction of these mutations in North Africa by Arab conquests was already discussed [41, 47, 48].

The eight found genotypes are responsible for intermediate phenotypes varying between the silent carrier and the classical trait due to the loss of one or two α- loci per chromosome. The rarity of hydrops fetalis and Hb-H disease in the studied population can be explained by the infrequency of the loss of three and four α- globin loci. Our work confirms previous observations describing the North African population as extremely heterogeneous [49]. The differences between the Moroccan and the Algerian and Tunisian α- thalassemia molecular spectrum are very notable and reflect the historical background differences of each country.

5. Conclusion

Our results have enriched the mutation spectrum of thalassemia in the north of Morocco, which has provided necessary information for its diagnosis. Carriers’ detection, genetic counseling, prenatal diagnosis, and focused prevention programs usually lead to a fall in births of affected newborns. Thus, we highly recommend that people in Larache province with microcytic hypochromic anemia strongly benefit from a routine thalassemia screening.

Data Availability

The datasets analyzed during the current study will be available from the corresponding author on reasonable request.

Disclosure

Achraf Laghmich applied MLPA technique in his laboratory.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Acknowledgments

This work was supported by the Collaborative Research Program of the International Centre for Genetic Engineering Biotechnology [CRP/MOR09-01], Molecular Basis of Hemoglobinopathies in Morocco, and by the Excellence Program of Research of the National Center of Research in Science and Techniques in Morocco [CNRST/I002/033]. The authors express their thanks to Dr. Ouardani Karima, Pediatrician at Larache Provincial Hospital, and all the staff for their collaboration in sample collection and scientific advice. The authors wish to thank Pr. Manuel Valdivia, Cadiz University, Spain.

References

  1. E. Vichinsky, “Complexity of alpha thalassemia: growing health problem with new approaches to screening, diagnosis, and therapy,” Annals of the New York Academy of Sciences, vol. 1202, pp. 180–187, 2010. View at: Publisher Site | Google Scholar
  2. D. J. Weatherall, “Phenotype-genotype relationships in monogenic disease: lessons from the thalassaemias,” Nature Reviews Genetics, vol. 2, no. 4, pp. 245–255, 2001. View at: Publisher Site | Google Scholar
  3. C. L. Harteveld and D. R. Higgs, “α-thalassaemia,” Orphanet Journal of Rare Diseases, vol. 5, no. 1, article no 13, 2010. View at: Publisher Site | Google Scholar
  4. R. Galanello and A. Cao, “Alpha-thalassemia,” Genetics in Medicine, vol. 13, no. 2, pp. 83–88, 2011. View at: Publisher Site | Google Scholar
  5. A. E. S. Souza, G. L. Cardoso, S. Y. L. Takanashi, and J. F. Guerreiro, “α-Thalassemia (3.7 kb deletion) in a population from the Brazilian Amazon region: Santarém, Pará State,” Genetics and Molecular Research, vol. 8, no. 2, pp. 477–481, 2009. View at: Publisher Site | Google Scholar
  6. H. Srivorakun, G. Fucharoen, K. Sanchaisuriya, and S. Fucharoen, “Diagnosis of common hemoglobinopathies among South East Asian population using capillary isoelectric focusing system,” International Journal of Laboratory Hematology, vol. 39, no. 1, pp. 101–111, 2017. View at: Publisher Site | Google Scholar
  7. F. B. Piel, A. J. Tatem, Z. Huang, S. Gupta, T. N. Williams, and D. J. Weatherall, “Global migration and the changing distribution of sickle haemoglobin: a quantitative study of temporal trends between 1960 and 2000,” The Lancet Global Health, vol. 2, no. 2, pp. e80–e89, 2014. View at: Publisher Site | Google Scholar
  8. J. Michlitsch, M. Azimi, C. Hoppe et al., “Newborn screening for hemoglobinopathies in California,” Pediatric Blood & Cancer, vol. 52, no. 4, pp. 486–490, 2009. View at: Publisher Site | Google Scholar
  9. J. Flint, A. V. Hill, D. K. Bowden et al., “High frequencies of α-thalassaemia are the result of natural selection by malaria,” Nature, vol. 321, no. 6072, pp. 744–750, 1986. View at: Publisher Site | Google Scholar
  10. K. Hafiani, H. Bazoui, Y. El Madhi et al., “Major sickle cell syndromes in children in Kenitra, Morocco,” Asian Pacific Journal of Tropical Disease, vol. 7, no. 11, pp. 688–690, 2017. View at: Publisher Site | Google Scholar
  11. H. Hardouz, H. Hami, A. Mokhtari et al., “Consanguineous marriages profile in the Tanger-Tetouan region in Morocco,” IOSR Journal of Pharmacy and Biological Sciences, vol. 9, no. 6, pp. 61–67, 2014. View at: Publisher Site | Google Scholar
  12. N. Agoumi and A. Sebar, “Les hémoglobinopathies au Maroc,” Archives de Pédiatrie, vol. 10, no. 7, pp. 648–657, 2003. View at: Publisher Site | Google Scholar
  13. “Haut commisariat au plan,” 2018, http://rgphencartes.hcp.ma/. View at: Google Scholar
  14. S. T. Singer, “Variable clinical phenotypes of alpha-thalassemia syndromes,” Scientific World Journal, vol. 9, pp. 615–625, 2009. View at: Google Scholar
  15. M. J. Rugless, C. A. Fisher, A. D. Stephens, R. J. Amos, T. Mohammed, and J. M. Old, “Hb Bart's in cord blood: an accurate indicator of α-thalassemia,” Hemoglobin, vol. 30, no. 1, pp. 57–62, 2006. View at: Publisher Site | Google Scholar
  16. F. P. L. Van der Dijs, M. Volmer, D. G. Van Gijssel-Wiersma, J. W. Smit, R. Van Veen, and F. A. J. Muskiet, “Predictive value of cord blood hematological indices and hemoglobin Barts for the detection of heterozygous alpha-thalassemia-2 in an African-Caribbean population,” Clinical Chemistry, vol. 45, no. 9, pp. 1495–1500, 1999. View at: Google Scholar
  17. C. Dodé, R. Krishnamoorthy, J. Lamb, and J. Rochette, “Rapid analysis of -α3.7 thalassaemia and αααanti3.7 triplication by enzymatic amplification analysis,” British Journal of Haematology, vol. 82, no. 1, pp. 105–111, 1993. View at: Publisher Site | Google Scholar
  18. C. L. Harteveld, A. Voskamp, M. Phylipsen et al., “Nine unknown rearrangements in 16p13.3 and 11p15.4 causing - and -thalassaemia characterised by high resolution multiplex ligation-dependent probe amplification,” Journal of Medical Genetics, vol. 42, no. 12, pp. 922–931, 2005. View at: Publisher Site | Google Scholar
  19. D. E. Sabath, “Molecular diagnosis of thalassemias and hemoglobinopathies,” American Journal of Clinical Pathology, vol. 148, no. 1, pp. 6–15, 2017. View at: Publisher Site | Google Scholar
  20. K. Kalaivani, “Prevalence & consequences of anaemia in pregnancy,” Indian Journal of Medical Research, vol. 130, no. 5, pp. 627–633, 2009. View at: Google Scholar
  21. M. El Hioui, AOT. Ahami, Y. Aboussaleh, J. D. Lemrini, and H. Loutfi, “Anemia in a Moroccan hospital setting: Typology and influence of scoio-demographic factors on its incidence,” Antropo, vol. 12, pp. 83–91, 2006. View at: Google Scholar
  22. E. B. Sellam and A. Bour, “Anemia among women of childbearing age in Morocco (Prefecture of Oujda- Angad),” Antropo, vol. 32, pp. 35–44, 2014. View at: Google Scholar
  23. H. Siala, F. Ouali, T. Messaoud, A. Bibi, and S. Fattoum, “α-Thalassaemia in Tunisia: some epidemiological and molecular data,” Journal of Genetics, vol. 87, no. 3, pp. 229–234, 2008. View at: Publisher Site | Google Scholar
  24. T. Henni, D. Bachir, P. Tabone, P. Jurdic, J. Godet, and P. Colonna, “Hemoglobin bart’s in northern Algeria,” Acta Haematologica, vol. 65, no. 4, pp. 240–246, 1981. View at: Publisher Site | Google Scholar
  25. M. Djembo-Taty, M. Tchiloemba, F. Galacteros, J. Rosa, and P. Lissouba, “Epidemiologic study of hemoglobinopathies in the Congo in 2,257 newborn infants,” Nouvelle revue francaise d"hematologie, vol. 28, no. 4, pp. 249–251, 1986. View at: Google Scholar
  26. A. M. Nhonoli, J. M. Kujwalile, P. W. Mmari, and Y. Shemaghoda, “Haemoglobin barts in newborn Tanzanians,” Acta Haematologica, vol. 61, no. 2, pp. 114–119, 1979. View at: Publisher Site | Google Scholar
  27. M. E. Pembrey, D. J. Weatherall, J. B. Clegg, C. Bunch, and R. P. Perrine, “Haemoglobin bart's in Saudi Arabia,” British Journal of Haematology, vol. 29, no. 2, pp. 221–234, 1975. View at: Publisher Site | Google Scholar
  28. D. R. Higgs, J. Lamb, B. E. Aldridge et al., “Inadequacy of Hb Bart’s as an indicator of alpha thalassaemia,” British Journal of Haematology, vol. 51, no. 1, pp. 177-178, 1982. View at: Publisher Site | Google Scholar
  29. T. Munkongdee, D. Pichanun, P. Butthep et al., “Quantitative analysis of Hb Bart's in cord blood by capillary electrophoresis system,” Annals of Hematology, vol. 90, no. 7, pp. 741–746, 2011. View at: Publisher Site | Google Scholar
  30. M. S. Hossain, E. Raheem, T. A. Sultana et al., “Thalassemias in South Asia: clinical lessons learnt from Bangladesh,” Orphanet Journal of Rare Diseases, vol. 12, no. 1, article no 93, 2017. View at: Publisher Site | Google Scholar
  31. S. Choudhari and M. K. Behera, “Chelation status & clinical profile of thalassemic children attending paediatric clinics,” Indian Journal of Applied Researc, vol. 6, Article ID 276278, pp. 276–278, 2016. View at: Google Scholar
  32. R. Zamani, S. Khazaei, and S. Rezaeian, “Survival analysis and its associated factors of beta thalassemia major in Hamadan province,” Iranian Journal of Medical Sciences, vol. 40, no. 3, pp. 233–239, 2015. View at: Google Scholar
  33. M. Bejaoui and N. Guirat, “Beta thalassemia major in a developing country: epidemiological, clinical and evolutionary aspects,” Mediterranean Journal of Hematology and Infectious Diseases, vol. 5, no. 1, Article ID e2013002, 2013. View at: Publisher Site | Google Scholar
  34. A. Inati, N. Zeineh, H. Isma'eel, S. Koussa, W. Gharzuddine, and A. Taher, “β-Thalassemia: the Lebanese experience,” Clinical & Laboratory Haematology, vol. 28, no. 4, pp. 217–227, 2006. View at: Publisher Site | Google Scholar
  35. C. K. Li, C. W. Luk, S. C. Ling, K. W. Chik, H. L. Yuen, C. K. Li et al., “Morbidity and mortality patterns of thalassaemia major patients in Hong Kong: retrospective study,” Hong Kong Medical Journal, vol. 8, no. 4, pp. 255–260, 2002. View at: Google Scholar
  36. K. M. Belhoul, M. L. Bakir, A. M. Kadhim, H. E. Dewedar, M. S. Eldin, and F. A. Alkhaja, “Prevalence of iron overload complications among patients with β-thalassemia major treated at Dubai thalassemia centre,” Annals of Saudi Medicine, vol. 33, no. 1, pp. 18–21, 2013. View at: Publisher Site | Google Scholar
  37. A. Zorai, C. L. Harteveld, A. Bakir et al., “Molecular spectrum of α –thalassemia in Tunisia: epidemiology and detection at birth,” Hemoglobin, vol. 26, no. 4, pp. 353–362, 2002. View at: Publisher Site | Google Scholar
  38. H. Mesbah-Amroun, F. Rouabhi, R. Ducrocq, and J. Elion, “Molecular basis of α-thalassemia in Algeria,” Hemoglobin, vol. 32, no. 3, pp. 273–278, 2008. View at: Publisher Site | Google Scholar
  39. H. A. Hamamy and N. A. S. Al-Allawi, “Epidemiological profile of common haemoglobinopathies in Arab countries,” Journal of Community Genetics, vol. 4, no. 2, pp. 147–167, 2013. View at: Publisher Site | Google Scholar
  40. K. Kyriacou, A. Kyrri, E. Kalogirou et al., “Hb Bart's levels in cord blood and α-thalassemia mutations in Cyprus,” Hemoglobin, vol. 24, no. 3, pp. 171–180, 2000. View at: Publisher Site | Google Scholar
  41. N. Jassim, S. Al-arrayed, and N. Gérard, “Molecular basis of a-thalassemia in Bahrain,” Bahrain Medical Bulletin, vol. 1, article no 23, 2001. View at: Google Scholar
  42. Y. Wee, K. Tan, T. W. Chow, S. Yap, and J. M. Tan, “Heterogeneity in alpha-thalassemia interactions in Malays, Chinese and Indians in Malaysia,” Journal of Obstetrics and Gynaecology Research, vol. 31, no. 6, pp. 540–546, 2005. View at: Publisher Site | Google Scholar
  43. N. O. Mota, E. M. Kimura, R. D. Ferreira et al., “Rare α0-thalassemia deletions detected by MLPA in five unrelated Brazilian patients,” Genetics and Molecular Biology, vol. 40, no. 4, pp. 768–773, 2017. View at: Publisher Site | Google Scholar
  44. A. H. Khelil, S. Denden, N. Leban et al., “Hemoglobinopathies in North Africa: a review,” Hemoglobin, vol. 34, no. 1, pp. 1–23, 2010. View at: Publisher Site | Google Scholar
  45. N. A. S. Al-Allawi, A. I. A. Badi, H. Imanian, N. Nikzat, J. M. S. Jubrael, and H. Najmabadi, “Molecular characterization of alpha-thalassemia in the Dohuk region of Iraq,” Hemoglobin, vol. 33, no. 1, pp. 37–44, 2009. View at: Publisher Site | Google Scholar
  46. S. El-Kalla and E. Baysal, “α-thalassemia in the United Arab Emirates,” Acta Haematologica, vol. 100, no. 1, pp. 49–53, 1998. View at: Publisher Site | Google Scholar
  47. C. L. Harteveld, M. Yavarian, A. Zorai, E. D. Quakkelaar, P. Van Delft, and P. C. Giordano, “Molecular spectrum of α-thalassemia in the Iranian population of hormozgan: three novel point mutation defects,” American Journal of Hematology, vol. 74, no. 2, pp. 99–103, 2003. View at: Publisher Site | Google Scholar
  48. A. Hellani, E. Fadel, S. El-Sadadi, H. El-Sweilam, A. El-Dawood, and K. K. Abu-Amero, “Molecular spectrum of alpha-thalassemia mutations in microcytic hypochromic anemia patients from Saudi Arabia.,” Genetic Testing and Molecular Biomarkers, vol. 13, no. 2, pp. 219–221, 2009. View at: Publisher Site | Google Scholar
  49. A. S. Teebi and T. Farag, Genetic Disorders among Arab Populations, Oxford University Press, New York, NY, USA, 2nd edition, 1997. View at: Publisher Site

Copyright © 2019 Achraf Laghmich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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