Polycystin-1 Inhibits Cell Proliferation through Phosphatase PP2A/B56α

Figure 3

Effects of overexpression of PC1 on B56α in cell lines. (a) The upper panel shows effects of WT PC1 on the expression of B56α. 100 μg of total protein from HEK293T cells stably expressing full-length PC1 or GFP vector was loaded for WB. The lower panel shows data representing the averaged level of B56α after normalization to β-actin from the upper panel. Shown is averaged B56α/β-actin (N = 5). β-Actin served as a loading control. (b) HeLa cells transiently expressing GFP-PC1-5TMC or GFP were performed as in Figure 3(a) (N = 5). (c) HEK293T cells transiently expressing GFP-PC1-5TMC or GFP were performed as in Figure 3(a) (N = 5).

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