Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant
In vitro plant regeneration from stem callus (3 times sub cultured) in R. tetraphylla. (a) Shoot bud induction from stem callus cultured on MS + TDZ (0.25 mg/L) + BAP (2 mg/L). (b) Multiple shoot buds transferred onto MS basal media. (c) Elongation of multiple shoot buds into small shoot lets on MS basal media. (d) Root induction in single shoot cultured on MS + IAA (1mg/L) + IBA (1 mg/L). (e) Root induction in single shoot cultured on MS + IAA (1mg/L) + IBA (1 mg/L). (f) Complete plantlet transferred to plastic cup containing sterile soil for hardening. (g) Field transfer (2 months old). (h) Acclimatized plant (24 months old).