Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Table 2

Effect of naphthalene acetic acid (NAA), on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.

Explant	MS + NAA (mg/L)	Morphogenetic Response	Callus
			Mean fresh weight (mg)	Mean dry weight (mg)
Leaf	1.0	Friable white callus	311.7 ± 0.31^c	19.1 ± 0.23^c
	2.0	Friable white callus	313.5 ± 0.23^d	19.5± 0.67^b
	3.0	Friable pale brown callus with roots	303.6 ± 0.55^e	19.0 ± 0.54^d
	4.0	Friable pale brown callus with roots	291.5 ± 0.18^f	17.2± 0.33^e
	5.0	Friable pale brown callus with roots	242.4 ± 0.23^j	15.6 ± 0.80^g
Stem; (Inter node)	1.0	Friable white callus	153.3 ± 0.14^l	2.2± 0.43^m
	2.0	Friable white callus	255.4 ± 0.40^i	4.9 ± 0.45^l
	3.0	Friable white callus	303.6 ± 0.47^e	10.1 ± 0.13^h
	4.0	Pale brown callus	343.1 ± 0.21^b	15.8 ± 0.36^f
	5.0	Pale brown callus	412.8 ± 0.49^a	25.1 ± 0.15^a
Root	1.0	White friable callus	256.5 ± 0.38^i	9.5 ± 0.19^k
	2.0	White friable callus	277.4 ± 0.14^g	16.1 ± 0.03^f
	3.0	Pale brown callus	262.1 ± 0.21^h	14.6 ± 0.43^h
	4.0	Brown callus	258.4 ± 0.74^i	13.9 ± 0.67^i
	5.0	Dark brown callus	243.8 ± 0.36^j	13.2 ± 0.31^i

Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.