Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant
Table 2
Effect of naphthalene acetic acid (NAA), on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.
Explant
MS + NAA (mg/L)
Morphogenetic Response
Callus
Mean fresh weight (mg)
Mean dry weight (mg)
Leaf
1.0
Friable white callus
311.7 ± 0.31c
19.1 ± 0.23c
2.0
Friable white callus
313.5 ± 0.23d
19.5± 0.67b
3.0
Friable pale brown callus with roots
303.6 ± 0.55e
19.0 ± 0.54d
4.0
Friable pale brown callus with roots
291.5 ± 0.18f
17.2± 0.33e
5.0
Friable pale brown callus with roots
242.4 ± 0.23j
15.6 ± 0.80g
Stem (Inter node)
1.0
Friable white callus
153.3 ± 0.14l
2.2± 0.43m
2.0
Friable white callus
255.4 ± 0.40i
4.9 ± 0.45l
3.0
Friable white callus
303.6 ± 0.47e
10.1 ± 0.13h
4.0
Pale brown callus
343.1 ± 0.21b
15.8 ± 0.36f
5.0
Pale brown callus
412.8 ± 0.49a
25.1 ± 0.15a
Root
1.0
White friable callus
256.5 ± 0.38i
9.5 ± 0.19k
2.0
White friable callus
277.4 ± 0.14g
16.1 ± 0.03f
3.0
Pale brown callus
262.1 ± 0.21h
14.6 ± 0.43h
4.0
Brown callus
258.4 ± 0.74i
13.9 ± 0.67i
5.0
Dark brown callus
243.8 ± 0.36j
13.2 ± 0.31i
Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.