Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant
Table 3
Effect of Kinetin (Kn) on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.
Explant
MS + Kn (mg/L)
Morphogenetic Response
Callus
Mean fresh weight (mg)
Mean dry weight (mg)
Leaf
1.0
No response
--
--
2.0
Friable white callus
201.4 ± 0.1
11.1 ± 0.1
3.0
Friable green callus
226.3 ± 0.4
13.5 ± 0.2
4.0
Pale brown callus
334.6 ± 1.0
18.5± 0.6
5.0
Pale brown callus
349.5 ± 0.4
22.8 ± 0.1
Stem (Inter node)
1.0
Friable white callus
218.6 ± 0.2
3.6 ± 0.1
2.0
Friable White callus
233.3 ± 0.2
4.2 ± 0.1
3.0
Friable White callus
264.9 ± 0.9
7.8 ± 0.2
4.0
Pale brown callus
309.4 ± 0.8
11.5 ± 0.6
5.0
Pale brown callus
343.2 ± 0.6
14.4 ± 0.2
Root
1.0
Single shoot bud
-
-
2.0
Two shoot buds
-
-
3.0
Multiple shoot buds
-
-
4.0
Multiple shoot buds
-
-
5.0
Multiple shoot buds
-
-
Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.
