Association Study of Myosin Heavy Chain 15 Polymorphisms with Asthma Susceptibility in Chinese Han

Figure 3

The allele-dependent dual-luciferase activity expression of MYH15 in HEK293 cells. Relative luciferase activity was measured in HEK293T cells transfected with firefly luciferase reporter plasmid pGL3-promoter containing each allele of the asthma-associated SNPs (rs9288876, rs7635009, and rs1454197). The pRL-CMV renilla luciferase reporter plasmid was cotransfected for normalization of transfection efficiency. The results are shown as relative fold changes in the constructed vector compared with the pGL3-promoter vector. Figure 3 I: the allele-dependent dual-luciferase activity expression of rs9288876 (A versus T: p<0.001, t-test). Figure 3 II: the allele-dependent dual-luciferase activity expression of rs7635009 (G versus A: p=0.001, t-test). Figure 3 III: the allele-dependent dual-luciferase activity expression of rs1454197 (G versus T: p<0.001, t-test). A: PGL3-promoter-A, T: PGL3-promoter-T, G: PGL3-promoter-G, Promoter: PGL3-promoter, and Basic: PGL3-basic.