Effect of Weightlessness on the 3D Structure Formation and Physiologic Function of Human Cancer Cells

Table 2

Publications (1997-2018) describing cell cycle alterations in human cancer cells under simulated microgravity.

Samples (cell line)	Exposure Devices	Speed (RPM^1)	Exposure time	Result	Ref.
Breast cancer
MDA-MB-231,MCF-7	s-μg / RCCS	20-25	5 d	S phase and G2/M phase increased	[40]
MCF-7	s-μg / RPM^2	—	48 h	slightly accumulate in G2/M	[43]
MDA-MB-231	s-μg / RPM^2	10	72 h	S phase increased after 24 h; while after 72 h S phase decline,	[41]
				G2/M phase increased
MCF-7	s-μg / MG-6C clinostat system	30	72 h	G2+M phase increased after 24 and 48 h	[42]
Other tumor
SGC-7901 gastric cancer	s-μg / MG-3 clinostat system	30	72 h	G0+G1 Phases increased significantly, while the S+G2+M phase	[44]
				decreased at 48, 72 h
H460 lung adenocarcinoma	s-μg / RPM^2	—	48 h	S phase increased, while G0/G1 phase decreased	[23]

Table 2. d, day; h, hour; RPM¹, rounds per minute; s-μg, simulated microgravity; RCCS: Rotational Cell Culture System; RPM²: Random Positioning Machine.