CXCR7 Inhibits Fibrosis via Wnt/β-Catenin Pathways during the Process of Angiogenesis in Human Umbilical Vein Endothelial Cells

Figure 4

CXCR7 activates ERK and AKT pathways but suppresses the Wnt/β-catenin pathway in HUVECs. (a) After cells upregulated or downregulated CXCR7 were treated with SDF-1(100 ng/ml), phosphorylation of ERK1/2 and AKT was detected by western blot analysis. (b) After cells upregulated or downregulated CXCR7 were treated with SDF-1(100 ng/ml), the expression of β-catenin and Wnt/β-catenin downstream target proteins c-Myc, survivin, and cyclinD1 were detected by western blot analysis. Relative protein levels were normalized to total ERK1/2, AKT, or GAPDH. si-NC: siRNA negative control group. oe-NC: overexpression negative control group. versus untreated control group, versus untreated control group, versus untreated control group, ^# versus SDF-1 group, ^## versus SDF-1 group, ^### versus SDF-1 group.

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