Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118

Figure 2

SDS-PAGE analysis of DEFB118 produced by E. coli Rosetta. (a) SDS-PAGE of DEFB118 from E. coli Rosetta. M protein markers (DL 150 kDa); Lane 1: E. coli Origami B (DE3)-pET32a (+) induced by 1 mmol/L IPTG for 10 h at 28°C; Lane 2: E. coli Origami B (DE3)-pET32a (+) induced by 1 mmol/L IPTG for 8 h at 28°C; Lane 3: E. coli Origami B (DE3)-pET32a (+) induced by 1 mmol/L IPTG for 6 h at 28°C; Lane 4: E. coli Origami B (DE3)-pET32a (+) induced by 1 mmol/L IPTG for 4 h at 28°C; Lane 5: E. coli Origami B (DE3)-pET32a (+) induced by 1 mmol/L IPTG for 2 h at 28°C; Lane 6: E. coli Origami B (DE3)-pET32a (+) (noninduced). (b) Purification of DEFB118. M protein markers (DL 150 kDa); Lanes 1-2 DEFB118 (1.2 mg/mL) purified by Ni 2+-IDA affinity chromatography; Lanes 3-5 DEFB118 (0.6 mg/mL) purified by Ni 2+-IDA affinity chromatography.

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