miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma

Figure 3

miR-876 regulates the expression of POSTN. (a) The prediction for miR-876 binding sites on POSTN transcript. (b) Schematic outlining the wild-type and mut-POSTN luciferase plasmid. (c) Luciferase activity in HEK-293 cells cotransfected with indicated miR-876 concentration or indicated POSTN luciferase reporter transcript. Data are showed as the ratio of firefly activity to Renilla luciferase activity. (d, e) The protein level of POSTN was measured by WB assays in indicated treated SMMC-7721 or HCC-LM3 cells. (f) The mRNA expression of POSTN was detected in indicated treated SMMC-7721 or HCC-LM3 cells by qRT-PCR. (g) The correlation analysis of POSTN and miR-876 in 127 tissues of HCC patients.

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