Lack of PPARβ/δ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis

<div>PPARβ/δ regulates the expression of SGK-1. (a) Western blot analysis of SGK-1 in PPARβ/δ-overexpressed HepG2 cells. (b, c) The mRNA levels of PPARβ/δ and SGK-1 were determined in shPPARβ/δ cells using qPCR, respectively. (d) Representative images of immunohistochemical staining from PPARβ/δ(-/-) mice and control mice, with a higher expression of SGK-1 in PPARβ/δ(-/-) mice. (e) ChIP-qPCR assays confirmed that the transcription factor PPARβ/δ can specifically bind to the regulatory region of SGK1 in HepG2 cells. 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(f) Whole-genome microarray analysis of gene expression in HepG2 cells transfected with PPARβ/δ_pEGFP-N1 or empty vector. Functional annotation was carried out in tabulation.</div>

BioMed Research International

fig4

Figure 4

Figure 4: Lack of PPARβ/δ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis