BioMed Research International

BioMed Research International / 2020 / Article

Research Article | Open Access

Volume 2020 |Article ID 9563851 |

Bo Shen, Aimin Li, Yu-Jui Yvonne Wan, Guijia Shen, Jinshui Zhu, Yuqiang Nie, "Lack of PPARβ/δ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis", BioMed Research International, vol. 2020, Article ID 9563851, 11 pages, 2020.

Lack of PPARβ/δ-Inactivated SGK-1 Is Implicated in Liver Carcinogenesis

Academic Editor: Brad Upham
Received19 May 2020
Accepted17 Aug 2020
Published05 Oct 2020


Objective. The present study examined the role of PPARβ/δ in hepatocellular carcinoma (HCC). Methods. The effect of PPARβ/δ on HCC development was analyzed using PPARβ/δ-overexpressed liver cancer cells and PPARβ/δ-knockout mouse models. Results. PPARβ/δ(-/-) mice were susceptible to diethylnitrosamine- (DEN-) induced HCC (87.5% vs. 37.5%, ). In addition, PPARβ/δ-overexpressed HepG2 cells had reduced proliferation, migration, and invasion capabilities accompanied by increased apoptosis and cell cycle arrest at the G0/G1 phase. Moreover, differential gene expression profiling uncovered that the levels of serine/threonine-protein kinase (SGK-1) mRNA and its encoded protein were reduced in PPARβ/δ-overexpressed HepG2 cells. Consistently, elevated SGK-1 levels were found in PPARβ/δ(-/-) mouse livers as well as PPARβ/δ-knockdown human SMMC-7721 HCC cells. Chromatin immunoprecipitation (ChIP) assays followed by real-time quantitative polymerase chain reaction (qPCR) assays further revealed the binding of PPARβ/δ to the SGK-1 regulatory region in HepG2 cells. Conclusions. Due to the known tumor-promoting effect of SGK1, the present data suggest that PPARβ/δ-deactivated SGK1 is a novel pathway for inhibiting liver carcinogenesis.

1. Introduction

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors, of which three isoforms exist: α, γ, and β/δ [13]. PPARβ/δ is the most widely expressed member of the PPAR family in human tissues and is abundantly found in the skin, intestine, and liver [4, 5]. PPARβ/δ is implicated in differentiation [6, 7], anti-inflammation [8], fatty acid catabolism [9], and preventing interleukin-6- (IL-6-) induced insulin resistance [10]. In animal models, PPARβ/δ agonists attenuate hepatic steatosis by enhancing fatty acid oxidation, reducing lipogenesis, and improving insulin sensitivity [ 11]. In humans, PPARβ/δ agonists reduce the hepatic fat content and elicit improvements in the plasma markers of liver function [12]. Furthermore, PPARβ/δ activation and overexpression inhibit lipogenesis in hepatocytes by increasing the expression of insulin-induced gene-1 [13].

Hepatocellular carcinoma (HCC) is one of the deadliest forms of cancer, and very limited data are available on the role of PPARβ/δ in HCC development. Studies have indicated that PPARβ/δ is a feasible target for chemoprevention in the last 10 years [14], although the functional outcomes of PPARβ/δ activation in some cancers are contradictory [15, 16]. However, the Human Protein Atlas database indicates that PPARβ/δ is undetectable in 80% of HCCs [14]. Nevertheless, it has been shown that PPARβ/δ activation promotes the proliferation and growth of human hepatic cancer cell lines through the upregulation of cyclooxygenase-2 (COX-2) and prostaglandin E2 production [17]. In contrast, another study has demonstrated that the COX-2 expression was not affected when human HCC cell lines were treated with PPARβ/δ ligands [18]. Therefore, the role of PPARβ/δ in hepatocarcinogenesis warrants further investigation. The aim of this study was to investigate the functional significance of PPARβ/δ in liver cancer cells and mouse models. Our data revealed the anti-HCC effect of PPARβ/δ and that PPARβ/δ-regulated serine/threonine-protein kinase (SGK-1) is implicated in the anti-HCC effect. In summary, PPARβ/δ-deactivated SGK-1 is a novel pathway for inhibiting tumor growth and linking metabolism and liver carcinogenesis together.

2. Materials and Methods

2.1. Experimental Animals and Study Design

PPARβ/δ-null mice in the C57BL/6 background were provided by Dr. Frank J. Gonzalez at the National Cancer Institute, National Institutes of Health, Bethesda, MD [19]. Genotyping was confirmed using the polymerase chain reaction (PCR), and animals were housed under controlled temperature () conditions with a 12 h light-dark cycle and were allowed free access to food and water. Wild-type or PPARβ/δ-null mice (male, 15 days old; 8 per group) were given a single intraperitoneal injection of diethylnitrosamine (DEN) (5 mg/kg body weight; Sigma Chemical Co., St. Louis, MO) [19]. The mice were anesthetized by chloroform and were sacrificed without fasting at the indicated time points. Blood was collected by cardiac puncture, and the livers were excised and weighed. The presence and dimensions of the surface nodules were evaluated and recorded. Each liver was cut into strips of 2–3 mm in thickness to examine the presence of macroscopically visible lesions. HCC was diagnosed by an experienced pathologist based on gross or histological examination. All of the animal experiments were conducted in accordance with the guidelines provided by the Animal Experimentation Ethics Committee of Guangzhou Medical University.

2.2. Human Liver Cancer Cell Culture

Five liver cancer cell lines, HepG2, Huh7, Hep3B, SMMC7721 (ATCC, Manassas, VA), and MHCC97H (Shanghai Institute of Biochemistry and Cell Biology, Shanghai, China), were maintained in Dulbecco’s modified Eagle’s medium, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Gibco, Gaithersburg, MD).

2.3. PPARβ/δ Expression and Transfection

The pEGFP-PPARβ/δ and pEGFP vectors were constructed by Genechem Co., Ltd. (Shanghai, China) and were used for transfection by Lipofectamine 2000 (Invitrogen, Carlsbad, CA). PPARβ/δ-overexpressed HepG2 cells were selected using 800 μg/mL G418 (Mpbio) after transfection for 48 h. The cell lines were named as HepG2_PPARβ/δ and HepG2_mock, respectively.

2.4. RNA Interference and Transfection

The SMMC-7721-NC and SMMC-7721-shPPARD cells were generated using lentiviral transduction of LV008-shPPARβ/δ (shPPARD) or control LV008 vectors (NC) (Forevergen. China) into SMMC-7721 cells, respectively, followed by selection of stable cell lines in puromycin (2 μg/mL). The sequence of shPPARD was 5-AACT CAGTGATATCATTGAGCCTAATTCAAGAGATTAGGCTCAATGATATCACGTTTTTTC-3.

2.5. RNA Extraction and Real-Time Quantitative PCR (qPCR)

Total RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA) and reverse-transcribed with oligo (dT) and M-MLV reverse transcriptase (Invitrogen). qPCR was performed with the GoTaq® qPCR Master Mix kit (Promega, A6002). The primer pairs were designed with Primer Premier 5, and the sequences were as follows: PPARβ, F 5-GGGCTTCCACTACGGTGTTCAT-3, R 5-TACTGGCACTTGTTGCGGTTCTT-3; SGK-1, F 5-CAAATAGAGGTTCAAGGGAT-3, R 5-TTAGGAGGCTTAGGTGGA-3; and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), F 5-GAGTCAACGGATTTGGTCGT-3, R 5-GACAAGCTTCCCGTTCTCAG-3. GAPDH was used to normalize the mRNA level.

2.6. Western Blotting

The cells were washed and lysed, and the clarified lysates were processed for western blot analysis. The extracted protein sample was separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes. The blots were blocked with 5% nonfat milk and incubated with specific primary antibodies against PPARβ/δ (1 : 500, Santa Cruz), SGK-1 (1 : 2000, Abcam, Cambridge, MA), and GAPDH (1 : 5000, Abcam). The proteins were then incubated with the secondary antibody (1 : 2000, Abcam) and detected by enhanced chemiluminescence (Amersham Corp., UK).

2.7. Immunohistochemical Analysis of SGK-1

The paraffin-embedded liver sections of PPARβ(-/-) and wild-type mice were analyzed by immunohistochemistry using the monoclonal antibody specific for SGK-1 (1 : 200, Abcam). Positive signals were visualized by diaminobenzidine and counterstained with hematoxylin. The immunostaining intensity was scored by an experienced pathologist as follows: 0, no staining; 1, mild staining; 2, moderate staining; and 3, strong staining. The percentage of positive cells was semiquantitatively scored as follows: 0, <5%; 1, 6–25%; 2, 26–50%; 3, 51–75%; and 4, >75%. The final immunoreactivity score was calculated by adding the intensity and percentage scores.

2.8. Colony Formation Assay

HepG2 cells were transfected with GV230-PPARβ/δ or an empty vector to the preseeded cells in 6-well plates at a density of 50, 100, or 200 cells per well. After 14 days of stationary culture, the cells were fixed with 70% ethanol and stained with crystal violet (Sigma, St. Louis, MO). Colonies with more than 50 cells/colony were counted under a microscope to calculate the rate of colony formation. All of the data were obtained from three independent experiments.

2.9. Cell Growth Assay

The cell viability of HepG2_PPARβ/δ and HepG2_mock cells was determined by the cell counting kit-8 (CCK-8; Beyotime) in a 96-well plate at a density of cells/well. The optical density was measured at different time points.

2.10. Cell Cycle and Apoptosis Analysis

Flow cytometry was used to observe the cell cycle distribution and apoptosis. HepG2_PPARβ/δ and HepG2_mock cells were incubated with 10% FBS for 24 h after a serum starvation period of 12 h. The cells were fixed in 70% ethanol and stained with 50 μg/mL propidium iodide (BD Pharmingen, San Jose, CA). Then, the cells were sorted by FACSCalibur (BD Biosciences, San Jose, CA), and the cell-cycle profiles were analyzed by the Flowjo software (Leonard A. Herzenberg, Stanford University, Palo Alto, CA). For apoptosis examination, HepG2_PPARβ/δ and HepG2_mock cells were stained with fluorescein isothiocyanate- (FITC-) conjugated annexin V and 7-amino-actinomycin, according to the manufacturer’s instructions (BD Biosciences).

2.11. Migration and Invasion Assays

The wound-healing assay was performed in vitro for cell migration analysis. Briefly, HepG2_PPARβ/δ and HepG2_mock cells ( cells/well) were cultured in 6-well plates until they reached 90% confluency [20]. Sterile tips were used to scratch the cell layers. Images of the wound closure areas were taken at 0, 24, and 48 h.

Matrigel migration and invasion assays were performed on HepG2_PPARβ/δ and HepG2_mock stably transfected liver cancer cells using 24-well Matrigel-biocoated migration and invasion chambers (Becton Dickinson, Waltham, MA), as previously described [21].

2.12. Microarray Analysis

The gene expression profiles of PPARβ/δ-overexpressed and empty vector-treated cells were obtained by oligonucleotide microarray analysis using an Illumina kit, according to the manufacturer’s instructions. Data were collected using the Illumina Genome Studio software. Functional annotation was carried out using gene lists submitted to a variety of online software tools, including the Database for Annotation, Visualization and Integrated Discovery (DAVID) [22] and Gene Set Enrichment Analysis (GSEA) [23].

2.13. Chromatin Immunoprecipitation (ChIP) Assay

ChIP assays were performed on HepG2 cells transfected with pEGFP-PPARβ/δ or pEGFP vectors (used as a control) using an EZ-Magna ChIP A kit (Millipore, Billerica, MA). The cells were cross-linked with 1% formaldehyde (Sigma-Aldrich) for 10 min and quenched by glycine. The cross-linked cells were collected in cold phosphate-buffered saline and sonicated to reduce the total DNA size to 200–1000 bp. The chromatin DNA fragments were precipitated overnight with 10 μg of PPARβ/δ antibody (Santa Cruz Biotechnology) or normal rabbit IgG at 4°C. The magnetic bead-antibody-chromatin complexes were washed, eluted, and incubated at 62°C for 2 h. The immunoprecipitated and input DNA was subjected to qPCR analysis using primers. The sequences of the SGK-1 promoter 1 were F 5-CAAATAGAGGTTCAAGGGAT-3 and R 5-TTAGGAGGCTTAGGTGGA-3.

3. Results

3.1. PPARβ/δ Deficiency Accelerates Hepatocarcinogenesis

The mice developed HCC induced by DEN at 8 months. DEN induced HCC in 37.5% (3/8) of the wild-type mice, while the prevalence of HCC was much higher in the PPARβ/δ(-/-) mice (87.5%, 7/8, ). Moreover, the average number of tumors per animal was 2.8-fold higher in the PPARβ/δ(-/-) mice compared with the wild-type mice (). Thus, PPARβ/δ deficiency increased the susceptibility of mice to DEN-induced hepatocarcinogenesis. No marked differences in the macroscopic or histological features of the HCCs were observed between the wild-type and PPARβ/δ-deficient mice, as evaluated by a pathologist (Figure 1).

3.2. Overexpression of PPARβ/δ Reduces Cell Proliferation and Induces Cell Cycle Arrest As Well As Apoptosis in HepG2 Cells

An elevated PPARβ/δ protein level was observed in human HCC SMMC7721 cells, while HepG2 and MHCC97H cells did not express PPARβ/δ protein (Figure 2(a)). Therefore, HepG2 cells were used for PPARβ/δ overexpression, and overexpression was confirmed by qRT-PCR and western blotting in HepG2 cells transfected with pEGFP-PPARβ/δ (Figures 2(b) and 2(c)).

The effect of PPARβ/δ overexpression on the cell viability of HepG2 cells was analyzed by the CCK-8 assay. The enhanced PPARβ/δ expression suppressed the cell viability in a time-dependent fashion (Figure 2(d)). The suppressive effect on cancer cell growth was further confirmed by the colony formation assay in stably transfected cells. The colony numbers of pEGFP-PPARβ/δ-transfected cells were reduced to 38% of that of the control cells (; Figure 2(e)). To further characterize the influence of PPARβ/δ on cell growth, flow cytometry was used to analyze the cell cycle distribution in HepG2 cells transfected with pEGFP-PPARβ/δ or control pEGFP vectors. We found that the overexpression of PPARβ/δ in HepG2 cells resulted in significant inhibition of cell cycle progression and the accumulation of G0–G1 phase cells ( vs. , Figure 2(f)). Cell apoptosis was determined by annexin V–FITC/propidium iodide fluorescence-activated cell sorting (FACS) analysis. The results showed an increase in the number of early apoptotic cells ( vs. , ) in HepG2 cells transfected with pEGFP-PPARβ/δ, as compared to the vector-transfected cells (Figure 2(g)).

3.3. Overexpression of PPARβ/δ Suppresses HepG2 Migration and Invasion

Wound-healing assays were conducted to evaluate migration in PPARβ/δ-overexpressed HepG2 cells. As shown in Figure 3(a), HepG2_mock cells spontaneously migrated and filled the wounded area within 48 h, while the migration of HepG2_PPARβ/δ cells was blocked or inhibited even after 48 h. In accordance with the results observed in the scratch assays, elevated expression of PPARβ/δ markedly attenuated the migration (, Figures 3(b) and 3(c)) and invasion of HepG2 cells (, Figures 3(d) and 3(e)) in the transwell migration and invasion assays. Taken together, these results indicate that PPARβ/δ is a potent suppressor of hepatoma cell migration and invasion.

3.4. PPARβ/δ Modulates the Expression Profiles of Cancer-Related Genes in HepG2 Cells

To elucidate the molecular mechanisms underlying the inhibitory effect of PPARβ/δ on HCC growth, the gene expression profiles in pEGFP-PPARβ/δ-transfected HepG2 cells were analyzed using whole-genome expression arrays from Illumina (humanHT-12_v4 beadchips). Principal component analysis utilizing the entire gene expression dataset showed the relatively tight clustering of the two groups and the clear separation of the experimental group from the control group. Compared with mock transfection, 222 upregulated and 382 downregulated genes were found in HepG2_PPARβ/δ cells. GSEA of the PPARβ/δ target genes revealed a significant drop in the average expression of genes related to metastasis and cell migration, cell adhesion, proliferation, angiogenesis, epithelial-to-mesenchymal transition, nuclear factor-κB, and transforming growth factor β signaling pathways, while upregulation in the average gene expression of cell cycle regulators (Figure 4(f)).

3.5. PPARβ/δ Transcriptionally Downregulates SGK-1 Expression

Expression array analysis indicated a 7.79-fold decrease in the abundance of SGK-1 expression in PPARβ/δ-overexpressed HepG2 cells. SGK-1 was one of the most downregulated genes. The downregulation of the SGK-1 expression by PPARβ/δ was confirmed by western blot (Figure 4(a)). The mRNA level of SGK-1 was noticeably increased when the PPARβ/δ activity was suppressed in SMMC-7721 cells infected with LV008-shPPARD (Figures 4(b) and 4(c)). A higher expression of SGK-1 protein was also detected in the livers of the PPARβ/δ(-/-) mice compared to that of the wild-type mice by immunohistochemistry (Figure 4(d)). These results indicated that PPARβ/δ might play a catalytic role through binding to the SGK-1 gene promoter. ChIP assays were performed on pEGFP-PPARβ/δ- or control vector-transfected HepG2 cells. Primarily, the transcription factor binding sites in the SGK-1 regulatory regions were evaluated using the JASPAR database (, and the PPARβ/δ recognition site (CCAGGCTAAAGTGCA) was found in the 5-regulatory region of the SGK-1 gene, which points to the role of the transcription factor PPARβ/δ in the expression of SGK-1. The immunoprecipitation was performed using an anti-PPARβ/δ antibody in chromatin DNA fragments, and a 163 bp fragment of the SGK-1 sequence was amplified from the immunoprecipitated DNA, indicating the direct binding of PPARβ/δ to SGK-1 (Figure 4(e)).

4. Discussion

Over the past decade, many studies have revealed the health benefits of PPARβ/δ in combating inflammation, lipogenesis, and insulin resistance. Activation of PPARβ/δ has been shown to have anticarcinogenic effects in skin cancer [24], pancreatic cancer [19], and prostate cancer [18], albeit not without controversy [15]. The role of PPARβ/δ in liver tumorigenesis has been established as well. Using a DEN-induced murine model of HCC, we demonstrated that a lack of PPARβ/δ increased the susceptibility to HCC formation. Our results were consistent with other studies using PPARβ/δ-knockout mice that showed an increased incidence of skin cancer [21], larger intestinal tumors [25], and chemically induced liver toxicity [23]. In addition, it has been reported that PPARβ/δ has an antiproliferative influence on prostate cancer cells, keratinocytes, and melanoma cells [24, 26, 27]. In order to investigate the effect of endogenous transactivation of PPARβ/δ in liver carcinogenesis, we examined its functional consequences by overexpressing PPARβ/δ in human HepG2 liver cancer cells. We found that the overexpression of PPARβ/δ resulted in inhibition of HepG2 cell proliferation in a time-dependent manner. The subsequent Hoechst staining and flow cytometry assays revealed that PPARβ/δ could induce apoptotic cell death and cell cycle arrest. Consistently, Coleman et al. have demonstrated that PPARβ/δ activation prevents the invasion and migration abilities of pancreatic cancer cells by activating the B cell lymphoma 6 pathway [19, 28]. Moreover, the current study revealed that overexpression of PPARβ/δ inhibited the liver cancer cell migration and invasion abilities.

It is well established that PPARβ/δ plays an important role in lipid and glucose metabolism and that it could be a potential molecule that links metabolism and carcinogenesis. The current study demonstrated by microarray analysis that SGK1, a member of the protein kinase A, G, and C families, is downregulated by PPARβ/δ. The immunohistochemistry results also supported this observation as the SGK-1 level was higher in PPARβ/δ-/- mice. Previous data have shown that PPARγ agonists induce the SGK-1 gene expression by direct binding [29]. The current study is the first to show that PPARβ/δ also regulates the SGK-1 gene expression but in a negative way. SGK-1 transcription is stimulated by excessive glucose levels and diabetes, oxidative stress, DNA damage, ischemia, neuronal injury, and a high-fat diet [3033]. In addition, active SGK-1 induces insulin release, adipocyte differentiation, and adipogenesis [31, 34]. The Human Protein Atlas database also shows elevated SGK-1 levels in liver cancer, colon cancer, myeloma, medulloblastoma, prostate cancer, ovarian tumors, and non-small-cell lung cancer [35]. Moreover, SGK-1-knockout mice are resistant to chemically induced colon carcinogenesis [31]. Recent findings also have shown that SGK-1 regulates cell survival, proliferation, and differentiation in several types of cancer cells such as kidney [31], breast [36], and liver cancer [37]. Additionally, SGK-1 may promote the survival of cholangiocarcinoma cells by mediating the IL-6-related pathway [38]. Furthermore, angiotensin II protects fibrosarcoma-derived cells from apoptosis by increasing SGK-1 phosphorylation [39]. Meanwhile, activated PPARβ/δ prevents IL-6-induced insulin resistance by inhibiting the signal transducer and activator of transcription 3 pathway in adipocytes, which was enhanced in PPARβ/δ-null mice [10]. Another study has suggested that PPARβ/δ protects against lipid accumulation and oxidative stress by reducing angiotensin II-induced activation of the Wnt signaling pathway [40]. Thus, through different signaling pathways, PPARβ/δ is implicated in metabolism and growth.

5. Conclusions

In conclusion, our data suggest that PPARβ/δ is a tumor suppressor in HCC and that downregulation of SGK-1 may be implicated in its tumor-suppressive effect.

Data Availability

The datasets generated and analyzed during the present study are available from the corresponding author on reasonable request.

Conflicts of Interest

The authors declare that they have no conflicts of interest.


The project was supported by National Natural Science Foundation of China (81270037) and the National Institutes of Health (CA222490).


  1. M. M. Aagaard, R. Siersbaek, and S. Mandrup, “Molecular basis for gene-specific transactivation by nuclear receptors,” Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, vol. 1812, no. 8, pp. 824–835, 2011. View at: Publisher Site | Google Scholar
  2. I. Issemann and S. Green, “Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators,” Nature, vol. 347, no. 6294, pp. 645–650, 1990. View at: Publisher Site | Google Scholar
  3. S. A. Kliewer, B. M. Forman, B. Blumberg et al., “Differential expression and activation of a family of murine peroxisome proliferator-activated receptors,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 15, pp. 7355–7359, 1994. View at: Publisher Site | Google Scholar
  4. E. E. Girroir, H. E. Hollingshead, P. He, B. Zhu, G. H. Perdew, and J. M. Peters, “Quantitative expression patterns of peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) protein in mice,” Biochemical and Biophysical Research Communications, vol. 371, no. 3, pp. 456–461, 2008. View at: Publisher Site | Google Scholar
  5. L. Berglund, E. Björling, P. Oksvold et al., “A genecentric Human Protein Atlas for expression profiles based on antibodies,” Molecular & Cellular Proteomics, vol. 7, no. 10, pp. 2019–2027, 2008. View at: Publisher Site | Google Scholar
  6. A. D. Burdick, D. J. Kim, M. A. Peraza, F. J. Gonzalez, and J. M. Peters, “The role of peroxisome proliferator-activated receptor-beta/delta in epithelial cell growth and differentiation,” Cellular Signalling, vol. 18, no. 1, pp. 9–20, 2006. View at: Publisher Site | Google Scholar
  7. J. M. Peters, Y. M. Shah, and F. J. Gonzalez, “The role of peroxisome proliferator-activated receptors in carcinogenesis and chemoprevention,” Nature Reviews Cancer, vol. 12, no. 3, pp. 181–195, 2012. View at: Publisher Site | Google Scholar
  8. K. S. Kilgore and A. N. Billin, “PPARbeta/delta ligands as modulators of the inflammatory response,” Current Opinion in Investigational Drugs, vol. 9, no. 5, pp. 463–469, 2008. View at: Google Scholar
  9. L. Salvadó, E. Barroso, A. M. Gómez-Foix et al., “PPARβ/δ prevents endoplasmic reticulum stress-associated inflammation and insulin resistance in skeletal muscle cells through an AMPK-dependent mechanism,” Diabetologia, vol. 57, no. 10, pp. 2126–2135, 2014. View at: Publisher Site | Google Scholar
  10. L. Serrano-Marco, R. Rodríguez-Calvo, I. El Kochairi et al., “Activation of peroxisome proliferator–activated receptor-β/-δ (PPAR-β/-δ) ameliorates insulin signaling and reduces SOCS3 levels by inhibiting STAT3 in interleukin-6–stimulated adipocytes,” Diabetes, vol. 60, no. 7, pp. 1990–1999, 2011. View at: Publisher Site | Google Scholar
  11. X. Palomer, E. Barroso, J. Pizarro-Delgado et al., “PPARβ/δ: a key therapeutic target in metabolic disorders,” International Journal of Molecular Sciences, vol. 19, no. 3, p. 913, 2018. View at: Publisher Site | Google Scholar
  12. H. E. Bays, S. Schwartz, T. Littlejohn III et al., “MBX-8025, a novel peroxisome proliferator Receptor-δ agonist: lipid and other metabolic effects in dyslipidemic overweight patients treated with and without atorvastatin,” The Journal of Clinical Endocrinology and Metabolism, vol. 96, no. 9, pp. 2889–2897, 2011. View at: Publisher Site | Google Scholar
  13. X. Qin, X. Xie, Y. Fan et al., “Peroxisome proliferator-activated receptor-delta induces insulin-induced gene-1 and suppresses hepatic lipogenesis in obese diabetic mice,” Hepatology, vol. 48, no. 2, pp. 432–441, 2008. View at: Publisher Site | Google Scholar
  14. J. M. Peters, P. L. Yao, and F. J. Gonzalez, “Targeting peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) for cancer chemoprevention,” Current Pharmacology Reports, vol. 1, no. 2, pp. 121–128, 2015. View at: Publisher Site | Google Scholar
  15. J. M. Peters, J. E. Foreman, and F. J. Gonzalez, “Dissecting the role of peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) in colon, breast, and lung carcinogenesis,” Cancer Metastasis Reviews, vol. 30, no. 3-4, pp. 619–640, 2011. View at: Publisher Site | Google Scholar
  16. D. Wang, L. Fu, W. Ning et al., “Peroxisome proliferator-activated receptor δ promotes colonic inflammation and tumor growth,” Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 19, pp. 7084–7089, 2014. View at: Publisher Site | Google Scholar
  17. B. Glinghammar, J. Skogsberg, A. Hamsten, and E. Ehrenborg, “PPARdelta activation induces COX-2 gene expression and cell proliferation in human hepatocellular carcinoma cells,” Biochemical and Biophysical Research Communications, vol. 308, no. 2, pp. 361–368, 2003. View at: Publisher Site | Google Scholar
  18. H. E. Hollingshead, R. L. Killins, M. G. Borland et al., “Peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) ligands do not potentiate growth of human cancer cell lines,” Carcinogenesis, vol. 28, no. 12, pp. 2641–2649, 2007. View at: Publisher Site | Google Scholar
  19. M. T. Bility, B. Zhu, B. H. Kang, F. J. Gonzalez, and J. M. Peters, “Ligand activation of peroxisome proliferator-activated receptor-β/δ and inhibition of cyclooxygenase-2 enhances inhibition of skin tumorigenesis,” Toxicological Sciences, vol. 113, no. 1, pp. 27–36, 2010. View at: Publisher Site | Google Scholar
  20. N. Martín-Martín, A. Zabala-Letona, S. Fernández-Ruiz et al., “PPARδ elicits ligand-independent repression of trefoil factor family to limit prostate cancer growth,” Cancer Research, vol. 78, no. 2, pp. 399–409, 2018. View at: Publisher Site | Google Scholar
  21. D. J. Kim, T. E. Akiyama, F. S. Harman et al., “Peroxisome proliferator-activated receptor β (δ)-dependent regulation of ubiquitin C expression contributes to attenuation of skin carcinogenesis,” The Journal of Biological Chemistry, vol. 279, no. 22, pp. 23719–23727, 2004. View at: Publisher Site | Google Scholar
  22. D. W. Huang, B. T. Sherman, and R. A. Lempicki, “Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources,” Nature Protocols, vol. 4, no. 1, pp. 44–57, 2009. View at: Publisher Site | Google Scholar
  23. W. Shan, C. J. Nicol, S. Ito et al., “Peroxisome proliferator-activated receptor-beta/delta protects against chemically induced liver toxicity in mice,” Hepatology, vol. 47, no. 1, pp. 225–235, 2008. View at: Publisher Site | Google Scholar
  24. M. G. Borland, P. L. Yao, E. M. Kehres et al., “Editor's highlight: PPARβ/δ and PPARγ inhibit melanoma tumorigenicity by modulating inflammation and apoptosis,” Toxicological Sciences, vol. 159, no. 2, pp. 436–448, 2017. View at: Publisher Site | Google Scholar
  25. R. Muller, “PPARβ/δ in human cancer,” Biochimie, vol. 136, pp. 90–99, 2017. View at: Publisher Site | Google Scholar
  26. J. M. Peters, F. J. Gonzalez, and R. Muller, “Establishing the role of PPARβ/δ in carcinogenesis,” Trends in Endocrinology and Metabolism, vol. 26, no. 11, pp. 595–607, 2015. View at: Publisher Site | Google Scholar
  27. R. L. Stephen, M. C. U. Gustafsson, M. Jarvis et al., “Activation of peroxisome proliferator-activated receptor δ stimulates the proliferation of human breast and prostate cancer cell lines,” Cancer Research, vol. 64, no. 9, pp. 3162–3170, 2004. View at: Publisher Site | Google Scholar
  28. J. D. Coleman, J. T. Thompson, R. W. Smith, B. Prokopczyk, and J. P. vanden Heuvel, “Role of peroxisome proliferator-activated receptorβ/δand B-cell lymphoma-6 in regulation of genes involved in metastasis and migration in pancreatic cancer cells,” PPAR Research, vol. 2013, Article ID 121956, 11 pages, 2013. View at: Publisher Site | Google Scholar
  29. G. Hong, A. Lockhart, B. Davis et al., “PPARγ activation enhances cell surface ENaCα via up-regulation of SGK1 in human collecting duct cells,” FASEB Journal, vol. 17, no. 13, pp. 1–17, 2003. View at: Publisher Site | Google Scholar
  30. K. Kitada, D. Nakano, Y. Liu et al., “Oxidative stress-induced glomerular mineralocorticoid receptor activation limits the benefit of salt reduction in Dahl salt-sensitive rats,” PLoS One, vol. 7, no. 7, article e41896, 2012. View at: Publisher Site | Google Scholar
  31. O. Nasir, K. Wang, M. Föller et al., “Relative resistance of SGK1 knockout mice against chemical carcinogenesis,” IUBMB Life, vol. 61, no. 7, pp. 768–776, 2009. View at: Publisher Site | Google Scholar
  32. D. Li, Z. Lu, J. Jia, Z. Zheng, and S. Lin, “Changes in microRNAs associated with podocytic adhesion damage under mechanical stress,” Journal of the Renin-Angiotensin-Aldosterone System, vol. 14, pp. 97–102, 2012. View at: Publisher Site | Google Scholar
  33. H. Tokuyama, S. Wakino, Y. Hara et al., “Role of mineralocorticoid receptor/Rho/Rho-kinase pathway in obesity-related renal injury,” International Journal of Obesity, vol. 36, no. 8, pp. 1062–1071, 2012. View at: Publisher Site | Google Scholar
  34. N. Di Pietro, V. Panel, S. Hayes et al., “Serum- and glucocorticoid-inducible kinase 1 (SGK1) regulates adipocyte differentiation via forkhead box O1,” Molecular Endocrinology, vol. 24, no. 2, pp. 370–380, 2010. View at: Publisher Site | Google Scholar
  35. F. Lang and C. Stournaras, “Serum and glucocorticoid inducible kinase, metabolic syndrome, inflammation, and tumor growth,” Hormones, vol. 12, no. 2, pp. 160–171, 2013. View at: Publisher Site | Google Scholar
  36. E. M. Sommer, H. Dry, D. Cross, S. Guichard, B. R. Davies, and D. R. Alessi, “Elevated SGK1 predicts resistance of breast cancer cells to Akt inhibitors,” The Biochemical Journal, vol. 452, no. 3, pp. 499–508, 2013. View at: Publisher Site | Google Scholar
  37. C. Talarico, L. D’Antona, D. Scumaci et al., “Preclinical model in HCC: the SGK1 kinase inhibitor SI113 blocks tumor progression in vitro and in vivo and synergizes with radiotherapy,” Oncotarget, vol. 6, no. 35, pp. 37511–37525, 2015. View at: Publisher Site | Google Scholar
  38. F. Meng, Y. Yamagiwa, S. Taffetani, J. Han, and T. Patel, “IL-6 activates serum and glucocorticoid kinase via p38α mitogen-activated protein kinase pathway,” American Journal of Physiology Cell Physiology, vol. 289, no. 4, pp. C971–C981, 2005. View at: Publisher Site | Google Scholar
  39. R. Baskin and P. P. Sayeski, “Angiotensin II mediates cell survival through upregulation and activation of the serum and glucocorticoid inducible kinase 1,” Cellular Signalling, vol. 24, no. 2, pp. 435–442, 2012. View at: Publisher Site | Google Scholar
  40. K. Sodhi, N. Puri, D. H. Kim et al., “PPARδ binding to heme oxygenase 1 promoter prevents angiotensin II-induced adipocyte dysfunction in Goldblatt hypertensive rats,” International Journal of Obesity, vol. 38, no. 3, pp. 456–465, 2014. View at: Publisher Site | Google Scholar

Copyright © 2020 Bo Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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