Circular RNA circPVT1 Contributes to Doxorubicin (DXR) Resistance of Osteosarcoma Cells by Regulating TRIAP1 via miR-137

Figure 1

circPVT1 was elevated in osteosarcoma tissues and cells. (a) RT-qPCR assay was employed to detect the expression level of circPVT1 in osteosarcoma tissues () and normal tissues (). (b) circPVT1 level was measured in the chemosensitive group (31) and the chemoresistant group (21). (c) Western blot assay was used to measure the protein levels of ABCB1 and MRP-1 in 52 osteosarcoma tissues and 45 normal tissues. (d) ABCB1 and MRP-1 protein levels were assessed in the chemosensitive group () and the chemoresistant group (). (e) Relative circPVT1 expression was examined in the human fetal osteoblastic cell line (hFOB1.19), parental osteosarcoma cells (KHOS and U2OS), and DXR-resistant osteosarcoma cells (KHOS/DXR and U2OS/DXR). (f) The protein levels of ABCB1 and MRP-1 were detected in parental osteosarcoma cells (KHOS and U2OS) and DXR-resistant osteosarcoma cells (KHOS/DXR and U2OS/DXR). .

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