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Behavioural Neurology
Volume 2015 (2015), Article ID 256196, 13 pages
Research Article

Feasibility and Safety of Continuous and Chronic Bilateral Deep Brain Stimulation of the Medial Forebrain Bundle in the Naïve Sprague-Dawley Rat

Laboratory of Stereotaxy and Interventional Neurosciences, Department of Stereotactic and Functional Neurosurgery, University Medical Center Freiburg, Breisacher Strasse 64, 79106 Freiburg, Germany

Received 12 January 2015; Accepted 29 March 2015

Academic Editor: Martin Bareš

Copyright © 2015 Luciano L. Furlanetti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The video clip presents SEEKING behavior induced by chronic continuous bilateral bipolar stimulation of the medial forebrain bundle in a rat, during the titration phase of our stimulation protocol. The video starts showing the animal's expected behavior in OFF condition. During the first few seconds of stimulation ON, visual fixation and freezing behavior can be observed, followed immediately by intensive sniffing and subsequent persistent explorative behavior of the home-cage and surrounding environment. The anti-TH micrographs enclosed in the video indicate the positioning of the stimulating electrodes in the MFB. MFB: Medial Forebrain Bundle; NAC: Nucleus Accumbens; CPu: Striatum; SNc: Substantia Nigra pars compacta; VTA: Ventral Tegmental Area; RRF: Retrorubral field. Yellow spots on the micrographs schematically represent the electric field within the stimulated area.

  1. Supplementary Material