Staurosporine Inhibits Frequency-Dependent Myofilament Desensitization in Intact Rabbit Cardiac Trabeculae
Figure 3
Figure 3 shows a representative acquisition and analysis of the force-calcium relationship. Panel (a) shows the chart of a potassium contracture. The trabecula in this example is twitching at 1 Hz (top of panel) when the superfusate solution is rapidly switched from normal Krebs Henseleit solution to that with a higher potassium and lower sodium concentration. Diastolic calcium (lower panel) is measured by rapidly switching the excitation wavelength between 340 and 380 nm. After the potassium solution is washed out, the contracture relaxes and the muscle recovers. Panels (b) and (c) show representative force pCa relationships obtained in trabeculae twitching at 1 or 4 Hz with vehicle (b) or staurosporine (c) in the superfusate solution.
