Changes in Activity and Kinetic Properties of the Proteasome in Different Rat Organs during Development and Maturation

Figure 3

Michaelis-Menten constants (𝐾_𝑚) of the proteasome in rat lens with age: Effect of aging on Michaelis-Menten constants of the proteasome in rat lens. 𝐾_𝑚-values were calculated as previously described (Materials and Methods, legend Figure 1). (a) Chymotrypsin-like activity: 𝑅²=0.008, 𝑃=.535. (b) Trypsin-like activity: 𝑅²=0.433, 𝑃<.0001. (c) Peptidylglutamyl peptidase activity: 𝑅²=0.242, 𝑃=.0004. A 𝑃-value of less than  .05 was considered statistically significant.

(a) Chymotrypsin-like activity (LLVY)

(b) Trypsin-like activity (VGR)

(c) Peptidylglutamyl-peptidase activity (LLE)