Identification of Functional Domain(s) of Fibrillarin Interacted with p2 of Rice stripe virus

Table 1

The sequences, homologous recombination, and restriction sites of PCR primers.

Primer and purpose	Sequence (5′→3′)^a	Modification
Construction for yeast two-hybrid assay
NS2-ADF	CGggatccTGATGGCATTACTCCTTTTCA	BamH1
NS2-ADR	CCGctcgagTCACATTAGAATAGGACACTCAT	Xho1
NbFib2-1-B DF	GgaattcATGGTTGCACCAACTAGAGGTCG	EcoR1
NbFib2-1-B DR	CGggatccTCAGGGATTCCACACTCTGTATTCAACCT	BamH1
NbFib2-2-B DF	GgaattcATGCCGTTCTAAGTTAGCAGCTGCAGTT	EcoR1
NbFib2-2-B DR	CGggatccTCACACCATCCCGACAAGCATTCTG	BamH1
NbFib2-3-B DF	GgaattcATGGCCTGATCAGGCAAGAATTTTAGC	EcoR1
NbFib2-3-B DR	CGggatccTCAGGCAGCAGCCTTCTGCTTCTT	BamH1
Construction for entry vector pDONR221
NbFib2-GF	ggggacaagtttgtacaaaaaagcaggcttcATGGTTGCACCAACTAGAGG	Homologous recombination
NS2-GF	ggggacaagtttgtacaaaaaagcaggcttcATGGCATTACTCCTTTTCA	Homologous recombination
NS2-GR	ggggaccactttgtacaagaaagctgggtcCATTAGAATAGGACACT	Homologous recombination
NbFib2-1-G F	ggggacaagtttgtacaaaaaagcaggcttcATGGTTGCACCAACTAGAGGTCG	Homologous recombination
NbFib2-1-G R	ggggaccactttgtacaagaaagctgggtcGGGATTCCACACTCTGTATTCAACCT	Homologous recombination
NbFib2-2-G F	ggggacaagtttgtacaaaaaagcaggcttcATGTTCCGTTCTAAGTTAGCAG	Homologous recombination
NbFib2-2-G R	ggggaccactttgtacaagaaagctgggtcCACCATCCCGACAAGCA	Homologous recombination
NbFib2-3-G F	ggggacaagtttgtacaaaaaagcaggcttcATGCCTGATCAGGCAAGAATT	Homologous recombination
NbFib2-3-G R	ggggaccactttgtacaagaaagctgggtcGGCAGCAGCCTTCTGCTTCTT	Homologous recombination

^aThe letters in lower case indicate homologous recombination sequence or a restriction enzyme site.