Hierarchical Mergence Approach to Cell Detection in Phase Contrast Microscopy Images

Figure 3

(a) The original phase contrast image is labeled as NO.100 in T24_xy1 image sequence of our datasets. (b) The merged binary map 𝐺_map. (c) The maximum binary ROI mask 𝑀_ROI. (d) The truncated image based on maximum ROI mask. (e) Blurred image based on Gaussian kernel. (f) Blurred image based on Figure 3(e) with gray morphological operations. (g) After applying Otsu method with three thresholds locally referring to Figure 3(f). (h) After applying watershed method to the blue section of Figure 3(g).

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