Research Article  Open Access
Machine Learning Approach to Automated Quality Identification of Human Induced Pluripotent Stem Cell Colony Images
Abstract
The focus of this research is on automated identification of the quality of human induced pluripotent stem cell (iPSC) colony images. iPS cell technology is a contemporary method by which the patient’s cells are reprogrammed back to stem cells and are differentiated to any cell type wanted. iPS cell technology will be used in future to patient specific drug screening, disease modeling, and tissue repairing, for instance. However, there are technical challenges before iPS cell technology can be used in practice and one of them is quality control of growing iPSC colonies which is currently done manually but is unfeasible solution in largescale cultures. The monitoring problem returns to image analysis and classification problem. In this paper, we tackle this problem using machine learning methods such as multiclass Support Vector Machines and several baseline methods together with Scaled Invariant Feature Transformation based features. We perform over 80 test arrangements and do a thorough parameter value search. The best accuracy (62.4%) for classification was obtained by using a NN classifier showing improved accuracy compared to earlier studies.
1. Introduction
Regenerative medicine is living a new era. Around ten years ago, Takahashi and Yamanaka demonstrated in [1] that mouse embryonic and adult fibroblasts can be reprogrammed back to stem cells by introducing four genes encoding transcription factors (Oct3/4, Sox2, Klf4, and cMYC [2]) [3]. Obtained stem cells were called induced pluripotent stem cells (iPSCs). One year later in an article by Takahashi et al. [4], it was shown that corresponding process can be repeated with human fibroblasts and the stem cells were called, respectively, human induced pluripotent stem cells (hiPSCs).
iPS cell technology has huge potential as Yamanaka has stated [3]. This fact is true not only because of the nature of iPSCs that they can be differentiated to any cell type wanted such as functional cardiomyocytes [5, 6] but also because they remove two major problems present with embryonic stem cells [3]:(1)Immune rejection after transplantation.(2)Severe ethical questions. Although iPS cell technology includes a lot of possibilities, there are still technical and biomedical challenges to be overcome such as teratoma formation and the uncertainty of nuclear reprogramming completeness of iPS cell clones before iPSC technology can be used, for example, for tissue repairing, disease modeling, and drug screening [3]. The use of iPSCs in disease modeling and drug screening is the most probable application to be achieved in the near future. Since derivation of iPSCs uses patient’s own cells, they are genetically identical and include all possible gene mutations of specific disease or condition which enables patient specific disease modeling and drug therapy [7].
Before we can apply human iPSCs as a standard method, there are still two major problems from the computational point of view which need to be solved. Currently, the quality monitoring of growing iPSC colonies is made manually. However, in the future when iPS cell colonies are grown in large scale, the quality controlling is impossible to carry out merely by human resources. Furthermore, only iPSC colonies of good quality should be identified and used in any further applications. Hence, the quality control process must be automated by taking, for example, an image with regular intervals from the colonies. This also gives an objective perspective to the decisionmaking.
The problem related to quality control returns to image analysis and classification tasks which can be divided into two separate questions:(1)In the reprogramming stage by using computational methods to identify when the patient’s somatic cells have been fully reprogrammed to iPSCs.(2)In the culturing stage to identify the quality of growing iPSC colonies in order to exclude the possible abnormal iPSC colonies which cannot be used in further measures. The first challenge was tackled in a recent article by Tokunaga et al. [8] where computational methods were examined regarding how to separate somatic cells and noniPSCs from the iPSCs. In the article wndchrm [9, 10], a multipurpose image classifier was applied to the image analysis and classification problems. However, the interest and the focus of this research are on the second challenge which can be divided into two different culture settings: (1)Automated quality identification of iPSC colony images where feeder cells are not included, that is, feederfree system.(2)Automated quality identification of iPSC colony images where feeder cells are included. Automated quality identification of stem cell colony images has been made also before. Jeffreys [11] used SVMs and texturalbased approach to classification. Nevertheless, the stem cells were not iPSCs since the publication was published before 2006. Furthermore, in an earlier report [12], stem cell classification was investigated using texture descriptors.
In our previous study [13], the feederfree system was examined whereas in the following reports [14, 15] the more challenging system with feeder has been analyzed. A common factor for our previous studies with feeders [14, 15] was that intensity histograms were used as a feature. In [14], baseline classification methods and one Directed Acyclic Graph Support Vector Machines (DAGSVM) [16] structure were used whereas in [15] the focus was on modified DAGSVM classification. In this current study, we address setting with feeders and compared to our previous researches [14, 15] we have included several new approaches to this paper including(1)larger image dataset,(2)new feature extraction method and a simple way to handle the features,(3)new classification methods.
More specifically, we use Scaled Invariant Feature Transformation (SIFT) [5, 17–20] instead of intensity histograms [14, 15, 21] and present a simple way to handle SIFT descriptors in classification problems. We perform over 80 test setups and thorough parameter value search. From the baseline classification methods, we use nearest neighbor (NN) classifier [14, 22–26] with different distance measures and distance weightings. Moreover, classification tree [25, 27, 28], linear discriminant analysis [23, 29–31], multinomial logistic regression [32, 33], and naïve Bayes [23, 28, 29, 34] with and without kernel smoothing density estimation [29] were used. Quadratic discriminant analysis [23] and discriminant analysis using Mahalanobis distance [35] were tested but these could not be evaluated due to nonpositively definite covariance matrix. From multiclass SVMs, we tested DAGSVM [16, 36–40], oneversusall (OVA) [36, 37, 41–43], oneversusone (OVO) [36, 37, 41, 42, 44], and binary tree SVM [45, 46]. We repeated our experimental SVM tests with seven kernels and in binary tree SVMs and DAGSVM we tested all possible orders what can be constructed from our classes (good/semigood/bad) in a dataset. Furthermore, we used LeastSquares SVM [47–51] in all our tests.
The paper has the following structure. In Section 2, we describe briefly the theory of binary LeastSquares Support Vector Machine classifier and give a presentation of the multiclass extensions used. Since the baseline classification methods are well known, a reader can find the descriptions of the methods from the aforementioned references. Section 3 presents the data acquisition procedure as well as the description of image dataset. A thorough description of design of experiments from the feature extraction and classification procedure are given in Section 4, and Section 5 is for the results and their analysis. Finally, Section 6 is for discussion and conclusion.
2. Methods
2.1. LeastSquares Support Vector Machines
LeastSquares Support Vector Machine (LSSVM) is a reformulation from traditional Vapnik’s SVM [52–54] which is solved by means of quadratic programming. LSSVM was developed by Suykens and Vandewalle [48–50]. The starting point for derivation of LSSVM classifier is that we have a training set , where and . According to [15, 48–50], LSSVM optimization problem can be presented as follows:where inequality constraints (when comparing to Vapnik’s SVM) have been changed to equality constraints:where is an error variable for the th training example and is a nonlinear mapping into a higher dimensional feature space. The Lagrangian for the aforementioned optimization problem is now based on [15, 48–50]where Lagrangian multipliers can have positive and negative values due to (2). In order to find the optimal solution for (1), we need to define the conditions of optimality [15, 48–50]:
Variables and can be eliminated and setting , , , and properly (see [15, 48–50] for details) we end up to a set of linear equations:where , and is a kernel function [15, 48–50]. By solving and from (5), we obtain a classifier in the dual space:which is equivalent to Vapnik’s standard SVM formulation [48–50].
2.2. Multiclass Extensions of LeastSquares Support Vector Machines
2.2.1. OneversusAll
Oneversusall (OVA) also known as oneversusrest is the first and the most intuitive approach to extend SVM to concern multiclass problems (the number of classes in classification problem is higher than two). Rifkin and Klautau [43] and Galar et al. [41] made an extensive overview to OVA method and in [48] LeastSquares SVM was extended to multiclass cases. The basic idea behind OVA is very simple. If we have an class problem, we train binary SVM classifiers where each one of them separates one class from the rest. An advantage of this approach is the low number of classifiers but disadvantages are possible ties and are that all classifiers are required to be trained using all training data.
For instance, in our research, we have three classes (bad/good/semigood or 1/2/3, resp.) and, thus, we construct three classifiers “1versusrest,” “2versusrest,” and “3versusrest” and the classifier which gives the positive output assigns the predicted class label for test example. If “1versusrest” classifier, for example, gives an output of and the rest of the classifiers give output “rest,” predicted class label will be for test example. However, this approach consists of a problem when two or more classifiers give positive output or all classifiers give output “rest” for test example. Thus, we end up to a tie situation. In these cases, a common practice is to apply the socalled winnertakesall principle where we need to compare the real outputs of OVA classifiers. In other words, we are looking forwhere denotes the th binary classifier which separates the class from the rest and is the test example. We used method given in [42] to solve possible ties. In [42], a NN classifier was trained with the training data of tied classes and a predicted class label was solved using trained NN classifier with Euclidean distance measure.
2.2.2. OneversusOne
Oneversusone (OVO) or pairwise coupling [36, 37, 41, 42, 47] is another commonly used SVM extension. If we again have an class classification problem, we construct a binary classifier for each class pair , where and when class labels are converted to numbers. Thus, we have altogether classifiers. A problem now becomes how to combine the results from individual binary SVM classifiers. Galar et al. [41] listed different methods for aggregations in OVO such as weighted voting strategy and nesting oneversusone method.
The most simplest way to obtain the predicted class label is to use majority voting [44] where we count the votes given by each classifier and assign predicted class label to be that class which has the most votes. However, like in OVA, voting is not always unambiguously determined and a tie might occur and some tie breaking rule must be applied. In this paper, we used, as in the case of OVA, a NN classifier as a tie solver. The actual tie breaking procedure goes as follows:(1)Collect the training data from classes that occurred in a tie.(2)Train a NN classifier using Euclidean metric and the training data obtained in step (1).(3)Classify a test example that occurred in a tie using the trained NN classifier in step (2).
2.2.3. DAGSVM
Directed Acyclic Graph Support Vector Machines (DAGSVMs) use Decision Directed Acyclic Graph (DDAG) structure. DAGSVM was introduced by Platt et al. [16]. There is a great similarity between DAGSVM and OVO since the training phase is the same for both methods. DDAG consists of nodes and each one of nodes has an SVM classifier [16, 38]. However, testing differs compared to OVO since it begins at the root node and continues via directed edges until a leaf node (predicted class label) is reached [38]. Altogether, comparisons are needed in the testing for an class classification task. When in OVO ties can be a problem, in DAGSVM ties are not a problem since onebyone classes are eliminated based on the decision of an SVM classifier.
One reason behind the development of DAGSVM was to tackle the problem related to unclassifiable regions where ties occur [41]. Although the ties are not anymore a problem in DAGSVM, a new problem is encountered. DDAG structure can be constructed with various ways and each one of them may produce different classification results [14] so the important question is which order is the best one from the classification point of view. In a general case when the number of classes is high, it is impossible in practice to test all possible orders [14, 38]. However, since we have only three classes in our dataset, it is possible to construct all the different orders and to determine the best choice for our application. Figures 1–3 show the DAGSVM structures what have been used in this study.
2.2.4. Binary Tree Support Vector Machines
Lorena et al. [55] made an extensive review on how to combine binary classifiers in multiclass problems. One possible solution is to use tree structures in classification. Compared to general DAG structures, trees have a simpler architecture and their use in classification tasks has gained popularity among practitioners and researchers. A central question, when trees are used in classification, is how to construct a tree. Lei and Govindaraju [56] used halfagainsthalf technique [57, 58] together with hierarchical clustering whereas Schwenker and Palm [46] applied confusion classes to define the binary partitions in a tree structure. Frank and Kramer [45, 55] stated that there are possible ways to construct a tree for a multiclass problem.
One possible binary tree structure is to reformulate oneversusall method. This approach was applied in [45, 59] with good results. Classification begins at the root node similarly as in DAGSVM and continues via directed edges. Each node eliminates one class from the classification and classifiers in nodes are trained by oneversusall principle. In the best case scenario, classification can end in the root node but the worst case scenario is that we need comparisons. The problem encountered in this approach is again the question in which order the classes should be eliminated in order to achieve the best possible classification performance. Fortunately, due to the low number of classes in our application, we were able to test all possible orders and the corresponding trees can bee seen from Figures 4–6. However, in a general class case where is very large, it is impossible to go through all possible orders computationally and some intelligent selection method must be applied.
3. Dataset
3.1. Image Data Acquisition
Human induced pluripotent stem cells were used in this study and the colonies were photographed between days 5 and 7 of their weekly growth cycle [14, 15]. The reason behind the choice of days 5–7 is the requirement to gain better visualization of the iPSC colonies [14, 15]. The growing iPSC colonies were observed before taking the colony images. In observation of iPSCs, Nikon Eclipse TS100 inverted routine microscope with an attached heating plate was used [14, 15]. After visual observation, photographed iPSC colonies were categorized to one of the classes (good/semigood/bad) [14, 15]. In the imaging process, lighting and sharpness of an image were userdefined which might bring some variability between images [14, 15]. Nevertheless, the same expert took all the images which minimized the variability [14, 15]. Furthermore, settings were fixed during photographing sessions [14, 15].
Overall, images were taken during several sessions and it caused some minor differences in the images [14, 15]. Growing iPSC colonies were usually located in the center of the image, thus, giving the best visual condition [14, 15]. However, sometimes observed colony was located near the edge of the well which caused some distortion in the lightning [14, 15]. Image acquisition was performed using Imperx IGVB1620MKC000 camera which was mounted to the microscope and connected to a notebook equipped with JAI Camera Control Tool software [14, 15]. All the images were taken with (width height) resolution.
3.2. Dataset Description
The study was approved by the ethical committee of Pirkanmaa Hospital District (R08070). iPSC lines were established with retroviruses encoding for OCT4, SOX2, KLF4, and MYC as described earlier [4]. All the cell lines had been characterized for their karyotypes and pluripotency as described earlier [60]. The colonies were categorized as good quality if they had rounded shape, translucent even color, and defined edges. Semigood quality colonies presented changes in color and structure, but still with clear edges, while bad quality colonies had partially lost the edge structure, vacuole could occasionally be observed and areas of threedimensional structures were observed (see Figure 7).
(a) Good quality colonies
(b) Semigood quality colonies
(c) Bad quality colonies
An image dataset containing 173 images altogether was analyzed including colonies with good, semigood, and bad quality. Table 1 indicates the accurate information of frequencies and proportions of the classes in dataset. All image data are anonymous and cannot be attached to any specific patient. All the images have been taken by the same expert who also determined the true class label for each image as was explained in Section 3.1. Figure 7 shows two example images from each class.

4. Design of Experiments
4.1. Feature Extraction
Feature extraction from images has attracted researchers for a long time and there is a huge amount of research related to this topic. For instance, histograms are used in image classification (see [21]) and Local Binary Patterns [20, 61, 62] is a commonly used method. We used another wellknown feature extraction method called Scaled Invariant Feature Transformation (SIFT) which was developed by Lowe [17, 18, 20]. Lowe [17] presents four steps with the corresponding descriptions for computing the SIFT features and these steps are as follows:(1)The first step is scalespace extrema detection where possible interest points, which are invariant to scale and orientation, are detected [17]. Search is implemented using a differenceofGaussian function [17].(2)The second step is keypoint localization where a detailed model is fitted to the nearby data for location, scale, and ratio of principal curvatures [17]. With the help of this information, unstable keypoint candidates can be excluded [17].(3)The third step is orientation assignment. In this step, for each keypoint local image gradients are evaluated and based on this information all possible orientations are assigned for keypoints [17]. By this means, keypoints obtain rotational invariant property [17].(4)The last step is to compute keypoint descriptors. In the previous steps location, scale and orientation of keypoints have been found. Determination of descriptors is made by measuring the local image gradients at the fixed scale in the environment of each keypoint [17]. Finally, local gradients are transformed so that they are highly distinctive and still invariant as possible to any other possible change [17].
Overall, according to [17], “SIFT transforms image data into scaleinvariant coordinates relative to local features.” More details concerning all the aforementioned stages can be found in [17]. We used the Matlab implementation of VLFeat 0.9.18 [63] when extracting the SIFT frames and their corresponding descriptors from the images. We used the default values in extraction of SIFT features. Each one of the descriptors is described as a 128dimensional vector and the dimension comes from Lowe’s algorithm. After extracting SIFT descriptors from the images, a question arises what to do with them and how we can use them in classification. It is not unusual that, from an image, for instance, ten thousand descriptors are obtained and when we have a large image collection, the number of descriptors in total becomes very high. A common strategy is to apply bagoffeatures (BoF) [64–66] approach which originates from bagofwords strategy used in natural language processing and information retrieval. Csurka et al. [65] define four stages for image classification when BoF is applied to image classification. These stages are [64, 65](1)feature extraction and representation,(2)codebook construction,(3)BoF representation of images,(4)training of learning algorithm. From the stages, codebook construction is computationally tedious because in this phase the descriptors from the training set images are collected together and they are clustered using means algorithm or other clustering algorithms. Centroids of the clusters are codewords and with the use of centroids we can solve how many descriptors from each image belong to each of the clusters and, thus, we can obtain a histogram for every image what can be used in training of a learning algorithm. The next step is that from the images of a test set SIFT descriptors are extracted and they are clustered by means of centroids. Thus, a histogram presentation can be produced for the test set of images and they can be used in classification.
However, BoF approach has a significant weakness. Since the number of clusters define the number of features, we need to find the optimal value of . This again leads to a situation where we need to repeat the means algorithm several times and when the number of descriptors is hundreds of thousands or even millions we require a lot of computational power.
We present a simple way to bypass the clustering phase. Our procedure goes as follows:(1)Repeat steps (2)–(4) for all images.(2)Extract SIFT descriptors from an image.(3)Compute a mean SIFT descriptor from descriptors gained in step (2). By this means, one obtains one 128dimensional vector.(4)Use the average descriptor obtained in step (3) in classification as a feature vector. In other words, we evaluate a mean SIFT descriptor for each image and the obtained mean descriptor can be used in classification.
4.2. Classification Procedure
4.2.1. Performance Measures
We selected three performance measures for this paper. Firstly, we used true positive (TP) measure which indicates the number of correctly classified samples from specific class. Secondly, we used true positive rate (TPR) in percentage which tells the proportion how well the specific class was classified ( is the minimum and is the maximum). Thirdly, we applied accuracy (%) which is defined as a trace of confusion matrix divided by the sum of all elements in a confusion matrix and it shows the overall performance [14, 15].
4.2.2. Nearest Neighbor
nearest neighbor method (hereafter referred to as NN) [22, 24, 25, 67] is a commonly used baseline classification method. There are three main parameters to be considered when NN is used in classification and these are(1)the value of ,(2)distance measure,(3)distance weighting function. All of these parameters are userdefined and there is no universal rule how to define optimal parameter combination since they are data dependent. For this research, we selected when the total number of tested values was . The odd values were selected in order to decrease the probability of ties. If a tie in a NN classifier occurred, it was solved by a random choice between tied classes. A huge variety of distance measures are available in the literature and we tested seven of them. The chosen measures were as follows: (1)Chebyshev.(2)Cityblock (also known as Manhattan measure).(3)Correlation.(4)Cosine.(5)Euclidean.(6)Standardized Euclidean.(7)Spearman. And these are the same as used in [14]. To the last parameter, distance weighting function, we also applied the same alternatives as in [14] and these were as follows: (1)Equal weighting.(2)Inverse weighting.(3)Squared inverse weighting. From the parameters, distance and weighting function can be fixed but the optimal value must be estimated. Common method for value estimation is to use crossvalidation technique [23, 25] and the extreme variation of it is leaveoneout (LOO) method known to be suitable for rather small datasets as ours. In this paper, we performed NN classification using nested crossvalidation [68–71] and, more specifically, nested leaveoneout (NLOO) method which consists of a two inner loops where the outer loop is for model assessment and the inner loop is for model selection.
NLOO is the most timeconsuming variant for parameter value estimation but the advantage lies especially in the maximization of training set size which is valuable when we are dealing with relatively small datasets. Basically, with the help of NLOO, we can estimate optimal parameter values for all examples in a dataset separately. If we had a dataset with thousands or tens of thousands examples, we could simply use the holdout method and apply 10fold crossvalidation to a training set, for example, in order to find optimal parameter values. NLOO would be very inefficient to use in the case of “big data.” NLOO procedure used in this paper can be described with the following steps and notice that NLOO has the consequence that there might not be one specific optimal value for all examples but the value might vary:(1)Let , , and is the dataset.(2)Repeat the following steps for all . Let be the th example excluded from . Hence, the rest of samples form the training set .(3)Perform LOO procedure for with the following way:(a)Repeat steps (b)–(d) in each LOO round.(b)Do a score standardization for the smaller training set and scale the validation example using the values of and obtained from the smaller training set.(c)Train NN classifier using specific value and standardized training data obtained in step (b).(d)Predict the class label for the validation example using the trained NN classifier.(e)When the last LOO round has been performed, evaluate accuracy of for the tested value.(4)When LOO procedure has been repeated with all values in , select the value which obtained the highest accuracy.(5)When in step (4) the optimal value for has been found, do score standardization for and scale using and obtained from .(6)Train the NN classifier using and predict class label for .(7)When all , , have been predicted, evaluate performance measures specified in Section 4.2.1.
4.2.3. Multiclass Support Vector Machines
In Section 2.2, multiclass extensions of SVM were described and now we present the common classification procedure what was used in this paper. Support Vector Machines include parameters to be estimated like NN. However, the number of parameters depends on the kernel. We tested altogether seven kernels: the linear, polynomial kernels having degrees from to , and the Gaussian Radial Basis Function (RBF) kernel. A common parameter for all kernels is the regularization parameter (also known as “boxconstraint”) and for RBF there is also another parameter, , to be estimated which is the width of Gaussian function.
We decided that parameter value spaces were the same for and ; that is, . Thus, 31 parameter values were tested for kernels other than RBF and 961 combinations of were tested for the RBF kernel. We used LeastSquares Support Vector Machines [48–50] in our implementations of multiclass SVMs and the possible ties in OVA and OVO were solved by using the NN classifier with Euclidean measure as was done in [42]. We used the autoscale property in training of a binary SVM classifier which means that the training data of an SVM classifier was score standardized and in the testing phase every SVM classifier scales test/validation example based on and obtained from training data. The autoscaling property was used in order to have the consistent classification procedure for all classification methods. Furthermore, all SVM classifiers in a multiclass extension were trained with the same parameter values as in [37].
The actual classification and parameter value search was performed by using NLOO approach described as follows:(1)Let , , and is the dataset.(2)Repeat the following steps for all . Let be the th example excluded from . Hence, the rest of samples form the training set .(3)Perform LOO procedure for with the following way:(a)Repeat steps (b)(c) in each LOO round of .(b)Train individual SVM classifiers using the smaller training data, specified kernel function, and parameter value or combination in the case of RBF kernel.(c)Predict the class label for the validation example using trained SVM classifiers.(d)When the last LOO round has been performed, evaluate accuracy of for the tested parameter value (combination).(4)When LOO procedure has been repeated with all values of or combinations in , select the parameter value (combination) which obtained the highest accuracy.(5)When in step (4) the optimal or for has been found, train SVM classifiers again using full and predict the class label for .(6)When all , , have been predicted, evaluate performance measures specified in Section 4.2.1. The consequence of NLOO method is that we cannot find one specific parameter value or parameter value combination but the optimal values may vary in the case of each test example.
4.2.4. Other Classification Methods
Since the other classification methods (classification tree, linear discriminant analysis, naïve Bayes and its variants, and multinomial logistic regression) used in this research did not require any parameter value estimation, we were able to use simpler LOO approach in classification. We also tested quadratic discriminant analysis (QDA) [23] and discriminant analysis using Mahalanobis distance [35] but both of these methods could not be evaluated since some of the covariance matrices in a training set were not positively defined and positively definiteness is a requirement for calculating the inverse of a covariance matrix which is needed for QDA and Mahalanobis distance calculations. The classification procedure can be explained in detail with the following way:(1)Let , , and is the dataset.(2)Repeat the following steps for all . Let be the th example excluded from . Hence, the rest of samples form the training set .(3)Do a score standardization for and scale based on and obtained from .(4)Train a classifier using and predict the class label of using trained classifier.(5)When all , , have been predicted, evaluate performance measures specified in Section 4.2.1.
We made all the tests and implementations of multiclass extensions of LSSVM with Matlab 2014a together with Image Processing Toolbox, Parallel Computing Toolbox, and Statistics Toolbox. In SIFT feature extraction, we used VLFeat 0.9.18 [63].
5. Results
In the tables, we have emphasized the best result or results in a tie situation in order to facilitate the analysis for a reader. When analyzing the results, it is good to keep in mind that in our preliminary researches [14, 15] with smaller dataset the best accuracy was 55%.
OVA approach has been suggested to be as good as other multiclass methods when classifiers such as SVMs are properly tuned [43]. When looking into the OVA results in Table 2, we notice immediately two facts. Firstly, the RBF kernel obtained the highest accuracy (60.1%) and, secondly, semigood class was the most difficult class to be recognized since the TPRs were always lower compared to corresponding TPRs of classes good and bad. One possible reason behind the difficulty of classification of class semigood might be that semigood class can be considered as a transition phase and it might consist of very heterogeneous colonies. Some of the colonies may include only small changes and be very close to good colonies whereas some other colonies might be closer to class bad colonies depending on the situation. An answer to the other questions related to the performance of the RBF kernel might lie in the OVA strategy itself. When SVM classifiers are trained in OVA, we need to use all training data in every binary classifier which might complicate the separation of classes and, thus, the simpler kernels do not perform well. Overall, the linear, quadratic, and RBF kernels were the best choices when using OVA since they were the only ones which achieved above 50% accuracy. The best TPs and TPRs for each class were 25 (61.0%) (class bad with the RBF and 5th degree polynomial kernels), 51 (68.9%) (class good with the linear kernel), and 29 (50.0%) (class semigood with the RBF kernel).

The results of OVO in Table 2 differ from OVA results. Firstly, now in each class, the linear kernel was the best one obtaining the highest TPs and TPRs and gained the highest accuracy . The TPs and TPRs were 28 (68.3%) for class bad, 52 (70.3%) for class good, and 25 (43.1%) for class semigood. Other kernels with above accuracies were the 3rd degree polynomial and RBF kernels. Now, the results had slightly different trend compared to OVA results and it might be explained with the fact that in OVO SVM classifiers are trained only with training data of th and th classes whereas in OVA results all SVM classifiers are trained using a full training set.
Table 3 shows the results of DAGSVM with different structures and kernel functions. If we go through the results of different orders one by one, we notice similarities with the OVO results. This is, of course, natural since DAGSVM has the same training phase as OVO but the difference appears in testing. In structure 1 (see Figure 1), in the root node, classes bad and good were separated. From the accuracies in structure 1, the linear (), 3rd degree polynomial (), and RBF () kernel were the best ones as also in OVO results. TPRs above were achieved only by the linear kernel in the case of classes bad and good. Class semigood was usually the worst (the highest TPR was with 3rd degree polynomial kernel) recognized class, but when the degree of polynomial kernel function increased, TPRs in class semigood stayed relatively stable whereas in other classes TPRs dropped significantly.

In structure 2 (see Figure 2), where the root node separated classes bad and semigood from each other, the best results were improved compared to structure 1 results. Now, the best accuracies were gained by the same kernels as in OVO and structure 1 case. However, from structure 1, the accuracies of the linear (), 3rd degree polynomial (), and RBF () kernels were improved above 1%. Respectively, TPs and TPRs for the linear kernel were 29 (70.7%) (class bad), 54 (73.0%) (class good), and 24 (41.4%) (class semigood). For the RBF, the only difference compared to the linear kernel TPs or TPRs was in the case of class good where TP and TPR were 53 (71.6%). Moreover, in class semigood, TPRs were not so stable as in structure 1 within different kernels.
The last DAGSVM structure (see Figure 3) obtained the lowest maximum accuracy within all DAGSVM structures. In this structure, classes good and semigood were separated in root node. Nevertheless, the same kernels obtained the best results as in previous structures. The linear kernel achieved accuracy and the RBF accuracy, respectively. The quadratic and 3rd degree polynomial kernels yielded accuracy. From the TPRs, the only case where was exceeded was for class good with the linear kernel 52 (70.3%). Otherwise, all TPRs were left below . For classes bad and semigood, TPs and TPRs of the linear kernel were 27 (65.9%) and 23 (39.7%). Similarly, as in structure 2, semigood TPRs had a great dispersion between kernels. One reason behind this might be in the difference of class sizes of good and semigood. In structure 2 where the best accuracy was achieved, the balance between the class sizes in root node was also good.
Table 4 presents the results of binary tree SVMs of Figures 4–6. All structures represent OVA method described in a binary tree formulation. Structure 4 (see Figure 4) separated class bad from the rest of the classes in root node. Now, the highest accuracy () was achieved by the linear kernel again and the TPs and TPRs were 28 (68.3%) (class bad), 52 (70.3%) (class good), and 19 (32.8%) (class semigood). It must be noticed that the TP and TPR of the linear kernel in class bad were the highest among structures 4–6. Other kernels which reached the limit in accuracy were the quadratic and RBF kernels. The overall results of structure 4 are comparable with OVA results and reflect the difficulty of separating one class from the rest in this application. With many kernels, class good was recognized with the best TPR and this is somehow a natural situation since class good was the biggest class in a dataset. One obvious reason might also be that class good is just better separable class in the feature space than other classes.

For structure 5 (see Figure 5) where class good was separated from other classes in the root node, the results did not change a lot from structure 4 results. However, there are some interesting differences. Firstly, the highest accuracy () was gained using this structure and with the linear kernel. A noticeable detail is that this accuracy was the same as the topmost accuracy in OVA results. Secondly, the best TP () and TPR () of class good were yielded by the linear kernel within all structures 4–6. For the class semigood, which usually was the most difficult class to be classified, the same trend continued also when structure 5 was used. Thirdly, the highest TP(R) combination 26 (44.8%) was achieved by the 3rd degree polynomial kernel and the corresponding accuracy was . These are clear differences from structure 4 results. The RBF kernel was a runnerup as in many cases before.
For the last binary tree structure, structure 6 (see Figure 6), the general level of results was lower compared to structures 4 and 5 results. All the accuracies were left below and the best accuracy () was obtained by the RBF kernel and not by the linear kernel as usual. If we fix a kernel and look for the TPRs from all classes, we notice that the level of TPRs is more balanced in a bad way compared to many other cases before. This, however, affects directly the results decreasingly. Class good, for instance, was recognized below TPRs with all kernels whereas with other structures over and nearly TPRs were achieved. Moreover, in class bad, all TPRs were left below as well as in class semigood. Again, one reason behind the poor performance of structure 6 may be that in the root node we were separating class semigood from the other classes. Class semigood seems to be confusing class in our dataset and it is easily mixed up with the other classes. More details can be found from Table 4.
The move from multiclass SVMs into other classification methods did not bring any global improvement to the results. From the accuracies, naïve Bayes (NB) with and without kernel smoothing density achieved accuracy of which was the best one in Table 5. This, however, is not a satisfactory result. When considering the classwise TPs and TPRs, classification tree was the best alternative for classes bad and semigood having TP(R)s 20 (48.8%) and 19 (32.8%). For class good topmost TP(R) were obtained by the traditional NB and the results were 61 (82.4%). This was the first time when a TPR value reached above limit. An explanation for NB result in class good may be that NB is a classifier which uses probabilities in classification phase and class good is the largest class in a dataset so it has also the highest a priori probability.

Table 6 presents the results of NN classifiers with different distance measures and weighting combinations. Accuracy column shows that there are three accuracies above and these were gained by the Euclidean measure and equal weighting (), Euclidean measure with inverse weighting (), and standardized Euclidean measure with inverse weighting (). Accuracy of is the best one throughout all the classification methods used in this paper and it improved the accuracy of our previous researches [14, 15] around . However, the accuracy of was yielded using smaller dataset compared to dataset which is used in this paper, so the improvement is even more valuable. Overall, NN succeeded well in the classification. If we exclude two distance measure and weighting combinations, all other accuracies were above whereas several SVM results did not achieve above accuracy. A closer look to the TPs and TPRs reveals that Euclidean measure was also a good choice for classes bad and semigood since the topmost results 25 (61.0%) and 29 (50.0%) were achieved using it. For class good, cosine measure together with inverse weighting obtained the highest TP and TPR being 62 (83.8%). These were the best ones also in the whole paper. Table 6 also shows that class good was generally the best classified class in the dataset and class semigood was usually the most difficult class to be recognized.

6. Discussion and Conclusions
This study focused on automated identification of the quality of iPSC colony images. The classification task was a multiclass problem with three possible classes (good/semigood/bad) for the iPSC colony images. The motivation behind the paper is both practical and scientific. iPS cell technology will be in the near future a standard method in drug and toxicology screens in vitro and for creating disease models in culture [3]. In longterm perspective, iPS cell technology will probably be used also for tissue repairing and the possibilities of iPSC technology are enormous [3]. From the practical point of view, iPSCs cannot be exploited for future needs without the help of image analysis and classification techniques.
When iPSCs are differentiating, the growing process of colonies must have automated monitoring because of at least three reasons. Firstly, we need to ensure that the newly reprogrammed iPSC colonies have proper quality and structure. Secondly, the quality of the iPSC colonies after multiple passages must remain good without signs of spontaneous differentiation. Thirdly, when the number of growing iPSC colonies is large which means thousands or millions of colonies, it is impossible to manage the quality control manually and, hence, automation of this process is inevitable.
The aim of this paper was to give new perspective to this difficult identification problem by using SIFT descriptors in feature extraction and to present a simple way to handle these descriptors. Moreover, we wanted to give an extensive overview to different classification methods. A special focus was given on DAGSVMs and binary tree LSSVMs where the crucial question was to find the right order to construct the graph or tree since different orders might always give different results. As a result, different constructions gave different results and the differences were clear. Overall, we performed over 80 test arrangements and made thorough parameter value searches for SVMs and NN classifiers.
The best result was obtained by NN classifier with Euclidean measure and equal weighting having the accuracy of 62.4%. The accuracy itself might feel low but we have gained significant improvement from the earlier researches [14, 15] where a smaller image collection was examined and intensity histograms were used as a feature. Our earlier publications already showed how difficult problem this is and the differences between images and classes are very small.
Just by watching the best accuracy, it is obvious that more research is needed. At the current stage, classifier which performs with a bit over 60% accuracy can work as a decision supporting tool for personnel. However, in order to move largescale better accuracy must be obtained. When taking into account that this paper is a preliminary study with the extended dataset, we have gained promising results and are able to take next steps further in our research. Since with many classification methods class good was recognized well, it gives an idea that classes semigood and bad could be merged and the classification task would reduce to binary classification problem. This could be a good idea because in the end our aim from the practical point of view is to separate good colonies from the rest since good hiPSC colonies can only be used in applications.
Although we have obtained improvement to our results, we have still many open questions. From the classification perspective, artificial neural networks have not been used for this problem and that is why a thorough examination of different ANN learning algorithms is needed as in the previous work with benthic macroinvertebrate image classification [72]. An important question related to the simple descriptor handling method is the use of feature selection methods. An obvious continuation is to apply, for instance, Scatter method [73] to this averaged SIFT descriptor data and to examine how it affects classification results. Moreover, we need to examine other sophisticated feature selection methods.
An essential question related to feature extraction is to use other texture descriptors such as Local Binary Patterns, Local Intensity Order Patterns, dense SIFT, intensity histograms, and Histogram of Oriented Gradients, for iPSC colony classification problem. Moreover, BoF approach must be tested in the future with aforementioned textural descriptors and with normal SIFT descriptors. A good example on comparison of different texture descriptors can be found from [74, 75]. Although automated quality identification of human iPSC colony images has shown to be a real challenge for the computational methods, we have gained improvement and we are fully convinced that the technical challenges will be overcome in future research.
Competing Interests
The authors declare that there are no competing interest regarding the publication of this paper.
Acknowledgments
The first author is thankful for Ella and Georg Ehrnrooth Foundation and Oskar Öflund Foundation for the support.
References
 K. Takahashi and S. Yamanaka, “Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors,” Cell, vol. 126, no. 4, pp. 663–676, 2006. View at: Publisher Site  Google Scholar
 G. Amabile and A. Meissner, “Induced pluripotent stem cells: current progress and potential for regenerative medicine,” Trends in Molecular Medicine, vol. 15, no. 2, pp. 59–68, 2009. View at: Publisher Site  Google Scholar
 S. Yamanaka, “A fresh look at iPS cells,” Cell, vol. 137, no. 1, pp. 13–17, 2009. View at: Publisher Site  Google Scholar
 K. Takahashi, K. Tanabe, M. Ohnuki et al., “Induction of pluripotent stem cells from adult human fibroblasts by defined factors,” Cell, vol. 131, no. 5, pp. 861–872, 2007. View at: Publisher Site  Google Scholar
 J. Zhang, G. F. Wilson, A. G. Soerens et al., “Functional cardiomyocytes derived from human induced pluripotent stem cells,” Circulation Research, vol. 104, no. 4, pp. e30–e41, 2009. View at: Publisher Site  Google Scholar
 L. Zwi, O. Caspi, G. Arbel et al., “Cardiomyocyte differentiation of human induced pluripotent stem cells,” Circulation, vol. 120, no. 15, pp. 1513–1523, 2009. View at: Publisher Site  Google Scholar
 K. AaltoSetälä, B. R. Conklin, and B. Lo, “Obtaining consent for future research with induced pluripotent cells: opportunities and challenges,” PLoS Biology, vol. 7, no. 2, Article ID e1000042, 2009. View at: Publisher Site  Google Scholar
 K. Tokunaga, N. Saitoh, I. G. Goldberg et al., “Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells,” Scientific Reports, vol. 4, article 6996, pp. 1–7, 2014. View at: Publisher Site  Google Scholar
 N. Orlov, L. Shamir, T. Macura, J. Johnston, D. M. Eckley, and I. G. Goldberg, “WNDCHARM: multipurpose image classification using compound image transforms,” Pattern Recognition Letters, vol. 29, no. 11, pp. 1684–1693, 2008. View at: Publisher Site  Google Scholar
 L. Shamir, N. Orlov, D. M. Eckley, T. Macura, J. Johnston, and I. G. Goldberg, “Wndchrman open source utility for biological image analysis,” Source Code for Biology and Medicine, vol. 3, article 13, 2008. View at: Publisher Site  Google Scholar
 C. G. Jeffreys, Support vector machine and parametric waveletbased texture classification of stem cell images [M.S. thesis], Massachusetts Institute of Technology, Operations Research Center, Cambridge, Mass, USA, 2004.
 M. Paci, L. Nanni, A. Lahti, K. AaltoSetälä, J. Hyttinen, and S. Severi, “NonBinary coding for texture descriptors in subcellular and stem cell image classification,” Current Bioinformatics, vol. 8, no. 2, pp. 208–219, 2013. View at: Publisher Site  Google Scholar
 Y. Gizatdinova, J. Rasku, M. Haponen et al., “Investigating local spatiallyenhanced structural and textural descriptors for classification of iPSC colony images,” in Proceedings of the 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, pp. 3361–3365, Chicago, Ill, USA, August 2014. View at: Publisher Site  Google Scholar
 H. Joutsijoki, M. Haponen, I. Baldin et al., “Histogrambased classification of iPSC colony images using machine learning methods,” in Proceedings of the IEEE International Conference on Systems, Man and Cybernetics (SMC '14), pp. 2611–2617, October 2014. View at: Publisher Site  Google Scholar
 H. Joutsijoki, J. Rasku, M. Haponen et al., “Classification of iPSC colony images using hierarchical strategies with support vector machines,” in Proceedings of the 5th IEEE Symposium on Computational Intelligence and Data Mining (CIDM '14), pp. 86–92, IEEE, Orlando, Fla, USA, December 2014. View at: Publisher Site  Google Scholar
 J. C. Platt, N. Christiani, and J. ShaweTaylor, “Large margin DAGs for multiclass classification,” in Advances in Neural Information Processing Systems, pp. 547–553, 2000. View at: Google Scholar
 D. G. Lowe, “Distinctive image features from scaleinvariant keypoints,” International Journal of Computer Vision, vol. 60, no. 2, pp. 91–110, 2004. View at: Publisher Site  Google Scholar
 D. G. Lowe, “Object recognition from local scaleinvariant features,” in Proceedings of the 7th IEEE International Conference on Computer Vision (ICCV '99), vol. 2, pp. 1150–1157, Kerkyra, Greece, September 1999. View at: Publisher Site  Google Scholar
 M.E. Nilsback and A. Zisserman, “Automated flower classification over a large number of classes,” in Proceedings of the 6th Indian Conference on Computer Vision, Graphics & Image Processing (ICVGIP '08), pp. 722–729, December 2008. View at: Publisher Site  Google Scholar
 M. S. Nixon and A. S. Aguado, Feature Extraction & Image Processing for Computer Vision, Academic Press, Oxford, UK, 3rd edition, 2012.
 O. Chapelle, P. Haffner, and V. N. Vapnik, “Support vector machines for histogrambased image classification,” IEEE Transactions on Neural Networks, vol. 10, no. 5, pp. 1055–1064, 1999. View at: Publisher Site  Google Scholar
 L. Bottou, C. Cortes, J. Denker et al., “Comparison of classifier methods: a case study in handwritten digit recognition,” in Proceedings of the 12th IAPR International Conference on Pattern Recognition, pp. 77–82, Jerusalem, Israel, 1994. View at: Publisher Site  Google Scholar
 K. J. Cios, W. Pedrycz, R. W. Swiniarski, and L. A. Kurgan, Data Mining: A Knowledge Discovery Approach, Springer, New York, NY, USA, 2007.
 T. M. Cover and P. E. Hart, “Nearest neighbor pattern classification,” IEEE Transactions on Information Theory, vol. 13, no. 1, pp. 21–27, 1967. View at: Publisher Site  Google Scholar
 R. O. Duda, P. E. Hart, and D. G. Stork, Pattern Classification, John Wiley & Sons, New York, NY, USA, 2nd edition, 2001.
 V. Mitra, C.J. Wang, and S. Banerjee, “Text classification: a least square support vector machine approach,” Applied Soft Computing Journal, vol. 7, no. 3, pp. 908–914, 2007. View at: Publisher Site  Google Scholar
 H. R. Bittencourt and R. T. Clarke, “Use of classification and regression trees (CART) to classify remotelysensed digital images,” in Proceedings of the IEEE International Geoscience and Remote Sensing Symposium (IGARSS '03), vol. 6, pp. 3751–3753, July 2003. View at: Publisher Site  Google Scholar
 J. Huang, J. Lu, and C. X. Ling, “Comparing naive bayes, decision trees, and SVM with AUC and accuracy,” in Proceedings of the 3rd IEEE International Conference on Data Mining (ICDM '03), pp. 553–556, November 2003. View at: Google Scholar
 T. Hastie, R. Tibshirani, and J. Friedman, The Elements of Statistical Learning—Data Mining, Inference, and Prediction, Springer, New York, NY, USA, 2nd edition, 2009.
 T. Mar, S. Zaunseder, J. P. Martínez, M. Llamedo, and R. Poll, “Optimization of ECG classification by means of feature selection,” IEEE Transactions on Biomedical Engineering, vol. 58, no. 8, pp. 2168–2177, 2011. View at: Publisher Site  Google Scholar
 J. Xie and Z. Qiu, “The effect of imbalanced data sets on LDA: a theoretical and empirical analysis,” Pattern Recognition, vol. 40, no. 2, pp. 557–562, 2007. View at: Publisher Site  Google Scholar
 A. Agresti, Categorical Data Analysis, John Wiley & Sons, New York, NY, USA, 1990.
 A. P. de Barros, F. de Assis Tenório de Carvalho, and E. de Andrade Lima Neto, “A pattern classifier for intervalvalued data based on multinomial logistic regression model,” in Proceedings of the IEEE International Conference on Systems, Man, and Cybernetics (SMC '12), pp. 541–546, Seoul, South Korea, October 2012. View at: Publisher Site  Google Scholar
 D. D. Lewis, “Naive (Bayes) at forty: the independence assumption in information retrieval,” in Proceedings of the 10th European Conference on Machine Learning, Chemnitz, Germany, April 1998, vol. 1398 of Lecture Notes in Artificial Intelligence, pp. 4–15, Springer, Berlin, Germany, 1998. View at: Google Scholar
 Y. Zhung and J. Zhou, “A study on contentbased music classification,” in Proceedings of the 7th International Symposium on Signal Processing and Its Applications (ISSPA '03), pp. 113–116, July 2003. View at: Publisher Site  Google Scholar
 R. Debnath, N. Takahide, and H. Takahashi, “A decision based oneagainstone method for multiclass support vector machine,” Pattern Analysis and Applications, vol. 7, no. 2, pp. 164–175, 2004. View at: Google Scholar
 C.W. Hsu and C.J. Lin, “A comparison of methods for multiclass support vector machines,” IEEE Transactions on Neural Networks, vol. 13, no. 2, pp. 415–425, 2002. View at: Publisher Site  Google Scholar
 H. Joutsijoki and M. Juhola, “DAGSVM vs. DAGKNN: an experimental case study with benthic macroinvertebrate dataset,” in Machine Learning and Data Mining in Pattern Recognition, P. Perner, Ed., vol. 7376 of Lecture Notes in Computer Science, pp. 439–453, Springer, Berlin, Germany, 2012. View at: Publisher Site  Google Scholar
 M. Luckner, “Reducing number of classifiers in DAGSVM based on class similarity,” in Image Analysis and Processing—ICIAP 2011, G. Maino and G. L. Foresti, Eds., vol. 6978 of Lecture Notes in Computer Science, pp. 514–523, Springer, New York, NY, USA, 2011. View at: Publisher Site  Google Scholar
 J. Martínez, C. Iglesias, J. M. Matías, J. Taboada, and M. Araújo, “Solving the slate tile classification problem using a DAGSVM multiclassification algorithm based on SVM binary classifiers with a oneversusall approach,” Applied Mathematics and Computation, vol. 230, pp. 464–472, 2014. View at: Publisher Site  Google Scholar
 M. Galar, A. Fernández, E. Barrenechea, H. Bustince, and F. Herrera, “An overview of ensemble methods for binary classifiers in multiclass problems: experimental study on onevsone and onevsall schemes,” Pattern Recognition, vol. 44, no. 8, pp. 1761–1776, 2011. View at: Publisher Site  Google Scholar
 H. Joutsijoki and M. Juhola, “Comparing the onevsone and onevsall methods in benthic macroinvertebrate image classification,” in Proceedings of the 7th International Conference on Machine Learning and Data Mining, New York, NY, USA, AugustSeptember 2011, vol. 6871 of Lecture Notes in Artificial Intelligence, pp. 399–413, Springer, Berlin, Germany, 2011. View at: Google Scholar
 R. Rifkin and A. Klautau, “In defense of onevsall classification,” Journal of Machine Learning Research, vol. 5, pp. 101–141, 2004. View at: Google Scholar  Zentralblatt MATH
 U. Krebel, “Pairwise classification and support vector machines,” in Advances in Kernel Methods—Support Vector Learning, MIT Press, Cambridge, Mass, USA, 1999. View at: Google Scholar
 E. Frank and S. Kramer, “Ensembles of nested dichotomies for multiclass problems,” in Proceedings of the 21st International Conference on Machine Learning, pp. 305–312, 2004. View at: Google Scholar
 F. Schwenker and G. Palm, “Treestructured support vector machines for multiclass pattern recognition,” in Multiple Classifier Systems, J. Kittler and F. Roli, Eds., vol. 2096 of Lecture Notes in Computer Science, pp. 409–417, Springer, Berlin, Germany, 2001. View at: Publisher Site  Google Scholar
 T. Van Gestel, J. A. K. Suykens, B. Baesens et al., “Benchmarking least squares support vector machine classifiers,” Machine Learning, vol. 54, no. 1, pp. 5–32, 2004. View at: Publisher Site  Google Scholar
 J. A. K. Suykens and J. Vandewalle, “Least squares support vector machine classifiers,” Neural Processing Letters, vol. 9, no. 3, pp. 293–300, 1999. View at: Publisher Site  Google Scholar
 J. A. K. Suykens and J. Vandewalle, “Multiclass least squares support vector machines,” in Proceedings of the International Joint Conference on Neural Networks (IJCNN '99), pp. 900–903, Washington, DC, USA, July 1999. View at: Google Scholar
 J. A. K. Suykens, T. Van Gestel, J. De Brabanter, B. De Moor, and J. Vandewalle, Least Squares Support Vector Machines, World Scientific, River Edge, NJ, USA, 2002.
 E. D. Übeylï, “Least squares support vector machine employing modelbased methods coefficients for analysis of EEG signals,” Expert Systems with Applications, vol. 37, no. 1, pp. 233–239, 2010. View at: Publisher Site  Google Scholar
 S. Abe, Support Vector Machines for Pattern Recognition, Springer, London, UK, 2nd edition, 2010.
 C. Cortes and V. Vapnik, “Supportvector networks,” Machine Learning, vol. 20, no. 3, pp. 273–297, 1995. View at: Publisher Site  Google Scholar  Zentralblatt MATH
 V. N. Vapnik, The Nature of Statistical Learning Theory, Springer, New York, NY, USA, 2nd edition, 2000. View at: Publisher Site
 A. C. Lorena, A. C. P. L. De Carvalho, and J. M. P. Gama, “A review on the combination of binary classifiers in multiclass problems,” Artificial Intelligence Review, vol. 30, no. 1–4, pp. 19–37, 2008. View at: Publisher Site  Google Scholar
 H. Lei and V. Govindaraju, “HalfAgainstHalf multiclass support vector machines,” in Proceedings of the 6th International Workshop on Multiple Classifier Systems, Seaside, Calif, USA, June 2005, vol. 3541 of Lecture Notes in Computer Science, pp. 156–164, Springer, Berlin, Germany, 2005. View at: Google Scholar
 H. Joutsijoki, “Halfagainsthalf multiclass support vector machines in classification of benthic macroinvertebrate images,” in Proceedings of the International Conference on Computer and Information Science (ICCIS '12), pp. 414–419, IEEE, Kuala Lumpeu, Malaysia, June 2012. View at: Publisher Site  Google Scholar
 X. Zhang, Y. Yin, and H. Huang, “Halfagainsthalf multiclass text classification using progressive transductive support vector machine,” in Proceedings of the 1st International Conference on Information Science and Engineering (ICISE '09), pp. 866–869, IEEE, Nanjing, China, December 2009. View at: Publisher Site  Google Scholar
 X. Yang, Q. Yu, L. He, and T. Guo, “The oneagainstall partition based binary tree support vector machine algorithms for multiclass classification,” Neurocomputing, vol. 113, no. 3, pp. 1–7, 2013. View at: Publisher Site  Google Scholar
 A. L. Lahti, V. J. Kujala, H. Chapman et al., “Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture,” DMM Disease Models and Mechanisms, vol. 5, no. 2, pp. 220–230, 2012. View at: Publisher Site  Google Scholar
 T. Ojala, M. Pietikäinen, and D. Harwood, “A comparative study of texture measures with classification based on feature distributions,” Pattern Recognition, vol. 29, no. 1, pp. 51–59, 1996. View at: Publisher Site  Google Scholar
 T. Ojala, M. Pietikainen, and D. Harwood, “Performance evaluation of texture measures with classification based on Kullback discrimination of distributions,” in Proceedings of the 12th International Conference on Pattern Recognition, pp. 582–585, Jerusalem, Israel, 1994. View at: Publisher Site  Google Scholar
 A. Vedaldi and B. Fulkerson, “VLFeat: an open and portable library of computer vision algorithms,” 2008, http://www.vlfeat.org/. View at: Google Scholar
 A. CruzRoa, J. C. Caicedo, and F. A. González, “Visual pattern mining in histology image collections using bag of features,” Artificial Intelligence in Medicine, vol. 52, no. 2, pp. 91–106, 2011. View at: Publisher Site  Google Scholar
 G. Csurka, C. R. Dance, L. Fan, J. Willamowski, and C. Bray, “Visual categorization with bags of keypoints,” in Proceedings of the ECCV International Workshop on Statistical Learning in Computer Vision, 2004. View at: Google Scholar
 E. Nowak, F. Jurie, and B. Triggs, “Sampling strategies for bagoffeatures image classification,” in Computer Vision—ECCV 2006, A. Leonardis, H. Bischof, and A. Pinz, Eds., vol. 3954 of Lecture Notes in Computer Science, pp. 490–503, Springer, Berlin, Germany, 2006. View at: Publisher Site  Google Scholar
 C.L. Liu, K. Nakashima, H. Sako, and H. Fujisawa, “Handwritten digit recognition: benchmarking of stateoftheart techniques,” Pattern Recognition, vol. 36, no. 10, pp. 2271–2285, 2003. View at: Publisher Site  Google Scholar
 A. Statnikov, I. Tsamardinos, Y. Dosbayev, and C. F. Aliferis, “GEMS: a system for automated cancer diagnosis and biomarker discovery from microarray gene expression data,” International Journal of Medical Informatics, vol. 74, no. 78, pp. 491–503, 2005. View at: Publisher Site  Google Scholar
 A. Statnikov, L. Wang, and C. F. Aliferis, “A comprehensive comparison of random forests and support vector machines for microarraybased cancer classification,” BMC Bioinformatics, vol. 9, article 319, pp. 1–10, 2008. View at: Publisher Site  Google Scholar
 S. Varma and R. Simon, “Bias in error estimation when using crossvalidation for model selection,” BMC Bioinformatics, vol. 7, article 91, 2006. View at: Publisher Site  Google Scholar
 J. Wu, H. Liu, X. Duan et al., “Prediction of DNAbinding residues in proteins from amino acid sequences using a random forest model with a hybrid feature,” Bioinformatics, vol. 25, no. 1, pp. 30–35, 2009. View at: Publisher Site  Google Scholar
 H. Joutsijoki, K. Meissner, M. Gabbouj et al., “Evaluating the performance of artificial neural networks for the classification of freshwater benthic macroinvertebrates,” Ecological Informatics, vol. 20, pp. 1–12, 2014. View at: Publisher Site  Google Scholar
 M. Juhola and V. Siermala, “A scatter method for data and variable importance evaluation,” Integrated ComputerAided Engineering, vol. 19, no. 2, pp. 137–139, 2012. View at: Publisher Site  Google Scholar
 M. Paci, L. Nanni, and S. Severi, “An ensemble of classifiers based on different texture descriptors for texture classification,” Journal of King Saud UniversityScience, vol. 25, no. 3, pp. 235–244, 2013. View at: Publisher Site  Google Scholar
 J. Zhang, M. Marszałek, S. Lazebnik, and C. Schmid, “Local features and kernels for classification of texture and object categories: a comprehensive study,” International Journal of Computer Vision, vol. 73, no. 2, pp. 213–238, 2007. View at: Publisher Site  Google Scholar
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