Case Reports in Transplantation / 2013 / Article / Tab 2

Case Report

Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools

Table 2

(a) CDC- and AMS-ELISA-based pretransplant crossmatch results of the 43-year-old recipient with her completely HLA-matched cadaveric kidney donor. (b) Corresponding analyses for anti-HLA antibody screening/identification.
(a)

Serum sampleCDC-CM (#)AMS-ELISA-CM
PBL T-cell B-cell Class IClass II

10/2009 6 6 8neg.neg.
10/2010 6/8 6 8neg.neg.

neg.: negative; pos.: positive; #: NIH score system of the standard CDC-based crossmatch as a percent of positive/dead (red coloured) cells (%) as shown in Table 1(a).
(b)

Serum sampleScreening ELISA/DynaChip (Ø)CDC cell tray (PBL)
Class IClass II Class I / II

07/2009 neg. (Ø) neg. (Ø)n.d.
10/2009 neg. (Ø) neg. (Ø)pos. (68% PRA)
01/2010 neg.neg. n.d.
04/2010 neg.neg. n.d.
07/2010 neg. neg. n.d.
10/2010 neg. (Ø) neg. (Ø) pos. (61% PRA)

neg.: negative; pos.: positive; n.d.: not done; the CDC-based cell tray analysis was performed during the autumnal screening (i.e., once a year in accordance with the guidelines of the European Federation of Immunogenetics (EFI)) using the samples taken in October 2009 and October 2010; Ø: additionally performed DynaChip analyses in all cases exhibiting negative results in accordance with the screening ELISA.