A Leydig Cell Tumour in a Cat: Histological and Immunohistochemical Findings

Table 1

Primary antibodies for immunohistochemistry and reactivity of feline Leydig cell tumour.

Antibody	Type (clone)	Company	Dilution	Incubation	Antigen retrieval^a	Immunoreactivity of neoplastic cells
Cytokeratin	MM (AE1/AE3)	Santa Cruz	1 : 100	1 hour	MW	Negative
Vimentin	MM (V9)	Novocastra	1 : 100	1 hour	MW	Positive
Melan-A	MM (A103)	Ventana	prediluted	1 hour	MW	Positive
Calretinin	RP	Cell Marque	prediluted	1 hour	MW	Positive

MM: mouse monoclonal; RP: rabbit polyclonal; MW: microwave.
^aAntigen retrieval: microwaved in 0.01 M citrate buffer (pH 6.0) at 650 W for 4 minutes and at 350 W for 15 minutes.