Case Reports in Veterinary Medicine

Case Reports in Veterinary Medicine / 2013 / Article

Case Report | Open Access

Volume 2013 |Article ID 670398 |

Pietro Asproni, Francesca Millanta, Davide Lorenzi, Alessandro Poli, "A Leydig Cell Tumour in a Cat: Histological and Immunohistochemical Findings", Case Reports in Veterinary Medicine, vol. 2013, Article ID 670398, 3 pages, 2013.

A Leydig Cell Tumour in a Cat: Histological and Immunohistochemical Findings

Academic Editor: A. F. Koutinas
Received25 Feb 2013
Accepted24 Mar 2013
Published09 Apr 2013


A 13-year-old intact male cat was submitted to castration after the finding of the enlargement of the right testis during the clinical visit. Macroscopically, a nodule of 2 cm of diameter was observed on the cut surface of the enlarged testis. Histologically, the nodule was composed by polyhedral to elongated cells with a large, eosinophilic, and vacuolated cytoplasm and small, round, and dark nuclei. These cells were arranged in acinar structures and solid sheets. The tumour was diagnosed as a Leydig cell tumour. Immunohistochemical analysis revealed that neoplastic cells were vimentin, calretinin, and melan-A positive, whereas a lack of immunoreactivity to cytokeratins confirmed the diagnosis. To our knowledge, this is the first description of a feline Leydig cells tumour without any concurrent testicular neoplasm or in a nonretained testis.

1. Introduction

Leydig cell tumours are neoplasms originating from the interstitial Leydig cells of the testis. These tumours are common in dogs and more rarely reported in other species [1]. In cats, few cases of testicular tumors have been, up now, described [2, 3] including some Leydig cell tumours located in retained testis, in castrated cats, or with a concurrent testicular neoplasm [47]. Here, we describe for the first time a case of feline leydigioma occurring in a noncastrated cat and without testicular retention.

2. Case Presentation

At the clinical exam, a 13-year-old intact European shorthair male cat showed the increase in volume and firmness of the right testis, while the left one was atrophic. Both were normally located into the scrotum. Ultrasonographic investigations allowed to identify an hypoechogenic nodule of 2 cm of diameter in the enlarged testis. The cat did not present behavioral alterations as aggressiveness, feminization, or inappropriate urination. According to the owner’s will, the cat was submitted to surgery to remove both the gonads.

On gross evaluation, the mass of the right testis was yellow to brown-colored and firm and elastic at the palpation. The neoplasm was well circumscribed and clearly demarcated from the healthy testis. Tissue samples were fixed in 10% neutral buffered formalin and processed by routine methods for histological investigation. Four m thick sections were cut and stained with hematoxylin and eosin for microscopic examination. Further tissue sections were prepared for immunohistochemical staining. Immunohistochemistry was performed with the biotin-streptavidin-peroxidase method with primary antibodies against human pan-cytokeratins (CK), vimentin, melan-A, and calretinin. The chromogen was 3,3′-diaminobenzidine (ImmPACT DAB, Vector Laboratories, Burlingame, CA, USA) for all the antibodies (10 min at room temperature). Feline skin (for CK and vimentin), adrenal gland (for calretinin), and amelanotic melanoma (for melan-A) samples were used as positive control tissues. As negative control, the primary antibodies were replaced with nonimmune rabbit serum or replacing the primary monoclonal antibodies with a murine subclass matched unrelated antibodies. Immunohistochemical protocols and results are described in Table 1.

AntibodyType (clone)CompanyDilutionIncubationAntigen retrievalaImmunoreactivity of neoplastic cells

Cytokeratin MM (AE1/AE3)Santa Cruz 1 : 1001 hourMWNegative
VimentinMM (V9)Novocastra1 : 1001 hourMWPositive
Melan-AMM (A103)Ventanaprediluted1 hourMWPositive
CalretininRPCell Marque prediluted1 hourMWPositive

MM: mouse monoclonal; RP: rabbit polyclonal; MW: microwave.
aAntigen retrieval: microwaved in 0.01 M citrate buffer (pH 6.0) at 650 W for 4 minutes and at 350 W for 15 minutes.

Histological examination revealed the presence of solid sheets and acinar structures composed by polyhedral to elongated cells with a large, eosinophilic, and vacuolated cytoplasm and small, dark, and round nuclei (Figure 1(a)). These vacuoles were of different sizes and optically empty. Neoplastic cell sheets and acinar structures were surrounded by a fine supporting connective stroma in which vascular formations were often present. In some parts of the tumour, small hemorrhagic foci were observed. Mitotic figures were very rare and bipolar. Due to microscopic findings, the neoformation was diagnosed as a Leydig cell tumour.

Immunohistochemistry showed a cytoplasmic positivity of neoplastic cells to vimentin (Figure 1(b)) and the lack of immunoreactivity to CK. These cells showed a weak and focal calretinin expression (Figure 1(c)) and strong melan-A cytoplasmic positivity (Figure 1(d)). These immunohistochemical findings allowed to confirm the diagnosis of Leydig cells tumour.

3. Discussion

Leydig cell tumours are common in dogs [1], whereas in cats they are only rarely described. These reports concern interstitial tumours occurred in ectopic testes or in cats previously submitted to castration [47]. In one of these cases, the Leydig cells tumour was associated with a Sertoli cell tumour [5]. Considering these reports, the tumour here described is the first case of Leydig cell tumour affecting a normally located testis and without any concurrent neoplasm.

The immunohistochemical panel allowed to better characterize the nodule. Vimentin immunoreactivity of neoplastic cells and the lack of CK expression has been previously described by Miller and colleagues in a interstitial tumour associated with a Sertoli cell tumour in a castrated cat [5]. Calretinin has been reported to be a useful immunohistochemical marker for normal and neoplastic human Leydig cells [8, 9]. In the case here described, calretinin was expressed mainly with a weak intensity with some foci of strong expression, as yet reported by Miller and colleagues [5].

Melan-A expression in Leydig cells has been reported in human [9] and canine tumours [10]. Melan-A belongs to a group of melanocytic differentiation antigens and its clone A103 is used to detect steroid hormones producing cells, even Leydig cells. For this reason, the research of melan-A expression by immunohistochemistry may help to recognize normal and neoplastic Leydig cells. Furthermore, melan-A immunoreactivity could suggest the androgens production by this tumour that can lead to alterations in the behavior of the affected cat, with episodes of aggressiveness or inappropriate urination [57]. The cat bearing the tumour here described did not show these signs, but the previous articles reported this possibility.

In conclusion, this report describes a rare case of feline genital system tumour with a novel clinical onset and with a detailed histological and immunohistochemical characterization. On the basis of our findings, Leydig cell tumor may be included in the differential diagnosis of feline testicular pathologies.

Conflict of Interests

The authors declare that there is no conflict of interests.


  1. N. J. MacLachlan and P. C. Kennedy, “Tumors of the genital systems,” in Tumors in Domestic Animals, D. J. Meuten, Ed., pp. 547–567, Iowa State Press, Ames, Iowa, USA, 4th edition, 2002. View at: Google Scholar
  2. C. Benazzi, G. Sarli, and B. Brunetti, “Sertoli cell tumour in a cat,” Journal of Veterinary Medicine A, vol. 51, no. 3, pp. 124–126, 2004. View at: Publisher Site | Google Scholar
  3. N. Miyoshi, N. Yasuda, Y. Kamimura, M. Shinozaki, and T. Shimizu, “Teratoma in a feline unilateral cryptorchid testis,” Veterinary Pathology, vol. 38, no. 6, pp. 729–730, 2001. View at: Publisher Site | Google Scholar
  4. A. L. Doxsee, J. A. Yager, S. J. Best, and R. A. Foster, “Extratesticular interstitial and Sertoli cell tumors in previously neutered dogs and cats: a report of 17 cases,” The Canadian Veterinary Journal, vol. 47, no. 8, pp. 763–766, 2006. View at: Google Scholar
  5. M. A. Miller, S. E. Hartnett, and J. A. Ramos-Vara, “Interstitial cell tumor and Sertoli cell tumor in the testis of a cat,” Veterinary Pathology, vol. 44, no. 3, pp. 394–397, 2007. View at: Publisher Site | Google Scholar
  6. D. K. Rosen and J. L. Carpenter, “Functional ectopic interstitial cell tumor in a castrated male cat,” Journal of the American Veterinary Medical Association, vol. 202, no. 11, pp. 1865–1866, 1993. View at: Google Scholar
  7. A. R. Tucker and J. R. Smith, “Prostatic squamous metaplasia in a cat with interstitial cell neoplasia in a retained testis,” Veterinary Pathology, vol. 45, no. 6, pp. 905–909, 2008. View at: Publisher Site | Google Scholar
  8. D. Augusto, E. Leteurtre, A. De La Taille, B. Gosselin, and X. Leroy, “Calretinin: a valuable marker of normal and neoplastic Leydig cells of the testis,” Applied Immunohistochemistry and Molecular Morphology, vol. 10, no. 2, pp. 159–162, 2002. View at: Publisher Site | Google Scholar
  9. R. E. Emerson and T. M. Ulbright, “The use of immunohistochemistry in the differential diagnosis of tumors of the testis and paratestis,” Seminars in Diagnostic Pathology, vol. 22, no. 1, pp. 33–50, 2005. View at: Publisher Site | Google Scholar
  10. J. A. Ramos-Vara, M. E. Beissenherz, M. A. Miller et al., “Immunoreactivity of A103, an antibody to Melan A, in canine steroid-producing tissues and their tumors,” Journal of Veterinary Diagnostic Investigation, vol. 13, no. 4, pp. 328–332, 2001. View at: Google Scholar

Copyright © 2013 Pietro Asproni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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