MicroRNA-101-3p Suppresses mTOR and Causes Mitochondrial Fragmentation and Cell Degeneration in COPD

Figure 4

The effect of miR-101-3p and TNF-α on ASMC remodeling. (a) Western blots for fibronectin and α-SMA protein expression in ASMC of healthy nonsmokers (n = 3) treated with miR-101-3p mimics at 48 hours. (b) Western blots for fibronectin and α-SMA protein expression at 48 hours in COPD-ASMC (n = 3) treated with miR-101-3p mimics. (c) Image analysis of fibronectin expression in ASMC of healthy nonsmokers (control) and COPD, as shown in Figures 4(a) and 4(b). (d) Image analysis of α-SMA expression in ASMC of healthy nonsmokers (control) and COPD, as shown in Figures 4(a) and 4(b). (e) Western blots of TNF-α-induced fibronectin and α-SMA protein expression in ASMC of healthy nonsmokers (control). (f) Western blots of TNF-α-induced fibronectin and α-SMA protein expression in COPD-ASMC. (g) Image analysis of TNF-α-induced fibronectin expression in ASMC of healthy nonsmokers (control) and COPD, as shown in Figures 4(e) and 4(f). (h) Image analysis of TNF-α-induced α-SMA expression in ASMC of healthy nonsmokers (control) and COPD, as shown in Figures 4(e) and 4(f). Bar charts represent protein expression as mean ± S.E.M. All values represent comparison to untreated cells.

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